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SARS-CoV-2 Proteins and Antibodies for Lateral-flow-based Neutralization Assay

11-Nov-2021

GeneTex has collaborated with an outside lab to create the key recombinant reagents for development of a lateral-flow-based neutralization assay for SARS-CoV-2. These components are designed to allow detection of neutralizing antibodies in a sample by demonstrating decreased interaction between the viral spike protein receptor-binding domain (RBD) and the ACE2 receptor in a lateral flow assay (LFA) format (Figure 1). Since these antibodies and proteins are recombinant, they are easily scalable for commercial purposes.

Custom Antibodies


Figure 1. (A) SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins. The signal intensity of the test line (T) decreases due to competition by the neutralizing antibody, while the control line (C) demonstrates a valid test. (B) The design and structure of the assay and its components.
Neutralizing Antibody: Spike RBD antibody [HL1002] (GTX635791) / C Line: Detector: Colloidal gold-conjugated human ACE2 (ECD) protein (GTX135683-pro) / Capture: Anti-human ACE2 (ECD) antibody (GTX635897) / T Line: Detector: Colloidal gold-conjugated human ACE2 (ECD) protein (GTX135683-pro) / Capture: SARS-CoV-2 Spike RBD protein

 

Reagents for Neutralization Assay
Recombinant Abs Citation Support KOKD Validation Comparable Abs

 

  Product Name Applications Cat. No.
Citation Support Spike (ECD) protein, His tag (active) ELISA, Functional Assay, Sandwich ELISA GTX135972-pro
  Spike (ECD) protein, B.1.1.7 / Alpha variant, His tag (active) ELISA, Functional Assay GTX136059-pro
  Spike (ECD) protein, B.1.617.2 / Delta variant, His tag (active) ELISA, Functional Assay, Sandwich ELISA GTX136432-pro
  Spike RBD protein, His tag (active) ELISA, Functional Assay, Sandwich ELISA GTX136090-pro
  Spike RBD protein, B.1.1.7 / Alpha variant, His tag (active) ELISA, Functional Assay, Sandwich ELISA GTX136014-pro
  Spike RBD protein, B.1.617.2 / Delta variant, His tag (active) ELISA, Functional Assay GTX136332-pro
  Spike RBD protein, AY.1 / Delta plus variant, His tag Functional Assay GTX136333-pro
Recombinant Abs Spike RBD antibody [HL1003-HU] Neutralizing/Inhibition, Sandwich ELISA GTX635866
Recombinant Abs Spike RBD antibody [HL1002] Neutralizing/Inhibition, Sandwich ELISA GTX635791
  Human ACE2 (ECD) protein, mouse IgG Fc tag (active) ELISA, Functional Assay GTX135683-pro
KOKD Validation Comparable Abs ACE2 antibody [GT19410] WB, IHC-P, ELISA, Sandwich ELISA GTX635897