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Influenza A and B Virus Recombinant Monoclonal Antibodies

09-Sep-2021

GeneTex has expanded its already extensive reagent catalog for influenza virus research with a series of recombinant rabbit monoclonal antibodies targeting the influenza A virus (FLUAV) and influenza B virus (FLUBV) nucleoproteins (NPs). These high-quality antibodies are validated for use in lateral flow assay (LFA; Figure 1 ), and the recommended LFA pairs are listed in Tables 1-2.

Influenza A and B Recombinant Monoclonal Antibodies


Figure 1. Detection of FLUAV and FLUBV NPs in lateral flow assays.
Recombinant NPs derived from influenza A virus (A) and influenza B virus (B) were detected by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1078: Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199); HL1089: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247); HL1103: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318); HL1068: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099); HL1069: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100); HL1073: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194); Sample: Influenza A virus Nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro); Sample: Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)

Table 1. The FLUAV Antibody Pair for Lateral Flow Assay

Detection Capture  
Clone HL1103 (GTX636318) Clone HL1089 (GTX636247)  

 

Table 2. The FLUBV Antibody Pair for Lateral Flow Assay

Detection Capture  
Clone HL1068 (GTX636099) Clone HL1069 (GTX636100)  

 

 

In addition, the antibodies are available as matched pairs for sandwich ELISA (sELISA) with sensitivities in the low picomolar range and superior EC50 values compared to other market-leading clones (Figure 2).


Figure 2. Detection of FLUAV and FLUBV NPs in ELISA assays.
Recombinant NPs derived from different strains of influenza A virus and influenza B virus were assayed by sELISA using (A) FLUAV NP antibodies GTX636318 and GTX636247 (as a pair), or (B) FLUBV NP antibodies GTX636099 and GTX636100 (as a pair). Indirect ELISA demonstrates the superior EC50 values of (C) FLUAV NP antibodies GTX636199, GTX636247, and GTX636318, as well as of (D) FLUBV NP antibodies GTX636100, GTX636099, and GTX636194, compared to a competitor’s clones.

 

Importantly, at least sixteen FLUAV strains and nine FLUBV strains can be detected by ELISA, western blot and immunocytochemistry, including FLUAV H1N1 and H3N2 subtypes (Figure 3). These recombinant antibodies showed higher sensitivities compared to the competitor’s clones (Figure 4). This data demonstrates the potential value of these recombinant antibodies for diagnostic applications.


Figure 3. Analysis of influenza virus strains.
Sandwich ELISA detects NPs in lysates from cells infected with different strains of (A) influenza A virus subtype H1N1 or (B) subtype H3N2 using FLUAV NP antibodies GTX636318 and GTX636247 (as a pair), or (C) influenza B virus using FLUBV NP antibodies GTX636099 and GTX636100 (as a pair). (D) The FLUAV NP antibody GTX636199 recognizes NPs of various FLUAV subtypes in infected-cell lysates by western blot. (E-F) Immunocytochemistry using FLUAV NP antibody GTX636247 (E) or FLUBV NP antibody GTX636194 (F) on NP gene-transfected 293T cells.

 

 


Figure 4. Comparison of GeneTex recombinant antibodies and market-leading clones.
Indirect ELISA assays exhibit the comparative sensitivities of (A) FLUAV NP antibodies GTX636318, GTX636247, and GTX636199, as well as of (B) FLUBV NP antibodies GTX636099, GTX636100, and GTX636194, compared to a competitor’s clones.

 

A complete list of applications is shown below for each featured recombinant protein and antibody (Tables 3-4).

Table 3. Featured recombinant proteins and antibodies for FLUAV research

  Product Name Clonality Applications Cat. No.
  Influenza A virus Nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag N/A ELISA GTX135903-pro
  Influenza A virus Nucleoprotein (A/California/2009 (H1N1)), DDDDK Tag N/A ELISA GTX135904-pro
  Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)), DDDDK Tag N/A ELISA GTX136317-pro
  Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag N/A ELISA, Lateral Flow, Sandwich ELISA GTX135868-pro
Influenza A virus Nucleoprotein antibody [GT1236] Ms mAb WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA GTX629633
Influenza A virus Nucleoprotein antibody [GT778] Ms mAb WB GTX629544
  Influenza A virus Nucleoprotein antibody [HL1078] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636199
  Influenza A virus Nucleoprotein antibody [HL1089] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636247
Influenza A virus Nucleoprotein antibody [HL1098] Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX636282
  Influenza A virus Nucleoprotein antibody [HL1103] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636318
Influenza A virus Nucleoprotein antibody Rb pAb WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA GTX125989
Influenza A virus H1N1 HA (Hemagglutinin) antibody Rb pAb WB, ICC/IF, IHC-P, ELISA GTX127357
  Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT223] Ms mAb WB, ICC/IF GTX629750
Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT521] Ms mAb WB GTX629746
Influenza A virus H1N1 NA (Neuraminidase) antibody Rb pAb WB GTX125974
Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] Ms mAb WB, ICC/IF GTX629696
Influenza A virus H3N2 HA (Hemagglutinin) antibody Rb pAb WB, IHC-P GTX127363
  Influenza A virus M1 (Matrix protein) antibody Rb pAb WB, ICC/IF GTX125928
Influenza A virus M1 (Matrix protein) antibody Rb pAb WB, ELISA GTX127356
Influenza A virus M2 (Matrix protein) antibody Rb pAb WB, ICC/IF GTX125951
  Influenza A virus NS1 (nonstructural protein) antibody Rb pAb WB, ICC/IF, IHC-P GTX125990
Influenza A virus NS1 (nonstructural protein) antibody [GT1653] Ms mAb WB GTX633685
  Influenza A virus NS2 / NEP antibody Rb pAb WB, ICC/IF GTX125953
Influenza A virus NS2 / NEP antibody Rb pAb WB GTX125952
Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX118991
Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX125932
Influenza A virus PB1 protein antibody Rb pAb WB, ICC/IF GTX125923
  Influenza A virus PB2 protein antibody Rb pAb WB, ICC/IF GTX125926

 

Table 4. Featured recombinant proteins and antibodies for FLUBV research

  Product Name Clonality Applications Cat. No.
  Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag N/A ELISA, Lateral Flow GTX135867-pro
  Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag N/A ELISA GTX136277-pro
  Influenza B virus Nucleoprotein antibody [HL1068] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636099
  Influenza B virus Nucleoprotein antibody [HL1069] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636100
  Influenza B virus Nucleoprotein antibody [HL1073] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636194
  Influenza B virus Nucleoprotein antibody [GT371] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629882
  Influenza B virus Nucleoprotein antibody [GT382] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629883
Influenza B virus Nucleoprotein antibody Rb pAb WB, ICC/IF, ELISA, Sandwich ELISA GTX128538
Influenza B virus Nucleoprotein antibody Rb pAb WB, ICC/IF, ELISA, Sandwich ELISA GTX128539
  Influenza B virus Nucleoprotein antibody Rb pAb WB, IHC-P (cell pellet) GTX135819
  Influenza B virus Hemagglutinin (HA) antibody Rb pAb WB, ICC/IF GTX128543
  Influenza B virus Hemagglutinin (HA) antibody Rb pAb WB, ICC/IF GTX128542
  Influenza B virus M (Matrix protein) antibody Rb pAb WB, ICC/IF GTX128537
  Influenza B virus M (Matrix protein) antibody Rb pAb WB GTX128536
  Influenza B virus Neuraminidase (NA) antibody Rb pAb ICC/IF GTX128541
  Influenza B virus Neuraminidase (NA) antibody Rb pAb ICC/IF GTX128540