GeneTex has expanded its already extensive reagent catalog for influenza virus research with a series of recombinant rabbit monoclonal antibodies targeting the influenza A virus (FLUAV) and influenza B virus (FLUBV) nucleoproteins (NPs). These high-quality antibodies are validated for use in lateral flow assay (LFA; Figure 1), and the recommended LFA pairs are listed in Tables 1-2.

Figure 1. Detection of FLUAV and FLUBV NPs in lateral flow assays.

Recombinant NPs derived from influenza A virus (A) and influenza B virus (B) were detected by lateral flow assay using the indicated recombinant rabbit monoclonal antibody pairs. HL1078: Influenza A virus Nucleoprotein antibody [HL1078] (GTX636199); HL1089: Influenza A virus Nucleoprotein antibody [HL1089] (GTX636247); HL1103: Influenza A virus Nucleoprotein antibody [HL1103] (GTX636318); HL1068: Influenza B virus Nucleoprotein antibody [HL1068] (GTX636099); HL1069: Influenza B virus Nucleoprotein antibody [HL1069] (GTX636100); HL1073: Influenza B virus Nucleoprotein antibody [HL1073] (GTX636194); Sample: Influenza A virus Nucleoprotein (NP) (A/WSN/1933 (H1N1)), DDDDK Tag (GTX135868-pro); Sample: Influenza B virus Nucleoprotein (NP) (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro). (C) Detection of FLUAV or FLUBV NPs by lateral flow assay is further confirmed using the indicated recombinant rabbit monoclonal antibody pairs.

 

Table 1. The FLUAV Antibody Pair for Lateral Flow Assay

Detection Capture Clone HL1103 (GTX636318) Clone HL1089 (GTX636247)

Table 2. The FLUBV Antibody Pair for Lateral Flow Assay

Detection Capture Clone HL1068 (GTX636099) Clone HL1069 (GTX636100)

In addition, the antibodies are available as matched pairs for sandwich ELISA (sELISA) with sensitivities in the low picomolar range and superior EC50 values compared to other market-leading clones (Figure 2).

Figure 2. Detection of FLUAV and FLUBV NPs in ELISA assays.

Recombinant NPs derived from different strains of influenza A virus and influenza B virus were assayed by sELISA using (A) FLUAV NP antibodies GTX636318 and GTX636247 (as a pair), or (B) FLUBV NP antibodies GTX636099 and GTX636100 (as a pair). Indirect ELISA demonstrates the superior EC50 values of (C) FLUAV NP antibodies GTX636199, GTX636247, and GTX636318, as well as of (D) FLUBV NP antibodies GTX636100, GTX636099, and GTX636194, compared to a competitor’s clones.

 

Importantly, at least sixteen FLUAV strains and nine FLUBV strains can be detected by ELISA, western blot and immunocytochemistry, including FLUAV H1N1 and H3N2 subtypes (Figure 3). These recombinant antibodies showed higher sensitivities compared to the competitor’s clones (Figure 4). This data demonstrates the potential value of these recombinant antibodies for diagnostic applications.

Figure 3. Analysis of influenza virus strains.

Sandwich ELISA detects NPs in lysates from cells infected with different strains of (A) influenza A virus subtype H1N1 or (B) subtype H3N2 using FLUAV NP antibodies GTX636318 and GTX636247 (as a pair), or (C) influenza B virus using FLUBV NP antibodies GTX636099 and GTX636100 (as a pair). (D) The FLUAV NP antibody GTX636199 recognizes NPs of various FLUAV subtypes in infected-cell lysates by western blot. (E-F) Immunocytochemistry using FLUAV NP antibody GTX636247 (E) or FLUBV NP antibody GTX636194 (F) on NP gene-transfected 293T cells.

Figure 4. Comparison of GeneTex recombinant antibodies and market-leading clones.

Indirect ELISA assays exhibit the comparative sensitivities of (A) FLUAV NP antibodies GTX636318, GTX636247, and GTX636199, as well as of (B) FLUBV NP antibodies GTX636099, GTX636100, and GTX636194, compared to a competitor’s clones.

 

A complete list of applications is shown below for each featured recombinant protein and antibody (Tables 3-4).

 

Table 3. Featured recombinant proteins and antibodies for FLUAV research

Product Name Clonality Applications Cat. No.   Influenza A virus Nucleoprotein (A/Kansas/2017/H3N2), DDDDK Tag   WB, ELISA, Lateral Flow, Sandwich ELISA GTX135903-pro   Influenza A virus Nucleoprotein (A/California/2009 (H1N1)), DDDDK Tag   WB, ELISA, Sandwich ELISA GTX135904-pro   Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)), DDDDK Tag   ELISA, Lateral Flow, Sandwich ELISA GTX136317-pro Influenza A virus Nucleoprotein (A/WSN/1933 (H1N1)), DDDDK Tag   ELISA, Lateral Flow, Sandwich ELISA GTX135868-pro  Influenza A virus Nucleoprotein antibody [GT1236] Ms mAb WB, ICC/IF, IHC-P, IHC-Fr, ELISA, Sandwich ELISA GTX629633 Influenza A virus Nucleoprotein antibody [GT778] Ms mAb WB GTX629544   Influenza A virus Nucleoprotein antibody [HL1078]  Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636199   Influenza A virus Nucleoprotein antibody [HL1089] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX636247 Influenza A virus Nucleoprotein antibody [HL1098]  Rb mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX636282   Influenza A virus Nucleoprotein antibody [HL1103] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet) GTX636318 Influenza A virus Nucleoprotein antibody [HL1321] Rb mAb WB GTX636739   Influenza A virus Nucleoprotein antibody [HL1323] Rb mAb WB, ICC/IF GTX636741   Influenza A virus Nucleoprotein antibody Rb pAb WB, ICC/IF, IHC-P, IP, ELISA, Sandwich ELISA GTX125989 Influenza A virus H1N1 HA (Hemagglutinin) antibody Rb pAb WB, ICC/IF, IHC-P, ELISA  GTX127357   Influenza A virus H1N1 HA (Hemagglutinin) antibody Rb pAb WB GTX127295   Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT223] Ms mAb WB, ICC/IF GTX629750 Influenza A virus H1N1 HA (Hemagglutinin) antibody [GT521] Ms mAb WB GTX629746 Influenza A virus H1N1 NA (Neuraminidase) antibody Rb pAb WB, ICC/IF GTX125974   Influenza A virus H1N1 NA (Neuraminidase) antibody [GT172] Ms mAb WB GTX629695 Influenza A virus H1N1 NA (Neuraminidase) antibody [GT288] Ms mAb WB, ICC/IF GTX629696 Influenza A virus H1N1 NA (Neuraminidase) antibody [HL1108] Rb mAb WB, ICC/IF GTX636323 Influenza A virus H3N2 HA (Hemagglutinin) antibody Rb pAb WB, IHC-P GTX127363 Influenza A virus H10N3 HA (Hemagglutinin) antibody [HL1559] Rb mAb ICC/IF, IHC-P (cell pellet) GTX637034   Influenza A virus M1 (Matrix protein) antibody Rb pAb WB, ICC/IF, IHC-Fr GTX125928 Influenza A virus M1 (Matrix protein) antibody Rb pAb WB, ELISA GTX127356 Influenza A virus M1 (matrix protein) antibody [HL1273] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636675 Influenza A virus M1 (matrix protein) antibody [HL1275] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636677 Influenza A virus M2 (Matrix protein) antibody Rb pAb WB, ICC/IF GTX125951   Influenza A virus NS1 (nonstructural protein) antibody Rb pAb WB, ICC/IF, IHC-P GTX125990 Influenza A virus NS1 (nonstructural protein) antibody [GT1653] Ms mAb WB GTX633685   Influenza A virus NS1 (nonstructural protein) antibody [GT1943] Ms mAb WB GTX633686 Influenza A virus NS1 (nonstructural protein) antibody [HL2130] Rb mAb WB GTX638102   Influenza A virus NS2 / NEP antibody Rb pAb WB, ICC/IF GTX125953 Influenza A virus NS2 / NEP antibody Rb pAb WB  GTX125952 Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX118991  Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX125932 Influenza A virus PA protein antibody Rb pAb WB, ICC/IF GTX125933 Influenza A virus PA protein antibody [HL1388] Rb mAb WB, ICC/IF GTX636828  Influenza A virus PB1 protein antibody Rb pAb WB, ICC/IF GTX125923 Influenza A virus PB1 protein antibody [HL1714] Rb mAb  WB, ICC/IF GTX637313 Influenza A virus PB1 protein antibody [HL1715] Rb mAb WB GTX637314 Influenza A virus PB2 protein antibody [HL1992] Rb mAb   WB, ICC/IF GTX637893   Influenza A virus PB2 protein antibody [HL1994] Rb mAb ICC/IF GTX637895   Influenza A virus PB2 protein antibody Rb pAb WB, ICC/IF  GTX125926 Influenza A virus PB2 protein antibody  Rb pAb  WB, ICC/IF  GTX125925

Table 4. Featured recombinant proteins and antibodies for FLUBV research

Product Name Clonality Applications Cat. No. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag   ELISA, Lateral Flow, Sandwich ELISA GTX135867-pro   Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag   ELISA, Lateral Flow, Sandwich ELISA GTX136277-pro Influenza B virus Nucleoprotein antibody [HL1068] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636099 Influenza B virus Nucleoprotein antibody [HL1069] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636100   Influenza B virus Nucleoprotein antibody [HL1073] Rb mAb WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA GTX636194   Influenza B virus Nucleoprotein antibody [GT371] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629882   Influenza B virus Nucleoprotein antibody [GT382]  Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629883 Influenza B virus Nucleoprotein antibody Rb pAb WB, ICC/IF, ELISA, Sandwich ELISA GTX128538  Influenza B virus Nucleoprotein antibody  Rb pAb WB, ICC/IF, ELISA, Sandwich ELISA GTX128539   Influenza B virus Nucleoprotein antibody Rb pAb WB, ICC/IF, IHC-P (cell pellet) GTX135819 Influenza B virus Hemagglutinin (HA) antibody [HL2208] Rb mAb WB GTX638212   Influenza B virus Hemagglutinin (HA) antibody Rb pAb WB, ICC/IF GTX128543 Influenza B virus Hemagglutinin (HA) antibody Rb pAb  WB, ICC/IF, ELISA GTX128542   Influenza B virus M (Matrix protein) antibody Rb pAb  WB, ICC/IF GTX128537   Influenza B virus M (Matrix protein) antibody  Rb pAb  WB  GTX128536   Influenza B virus Neuraminidase (NA) antibody  Rb pAb ICC/IF GTX128541   Influenza B virus Neuraminidase (NA) antibody  Rb pAb ICC/IF  GTX128540 Influenza B virus Neuraminidase (NA) antibody [HL1476] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636950   Influenza B virus antibody Rb pAb WB   GTX128544

Table 5. Other recombinant proteins and antibodies for FLU research

Product Name Clonality Applications Cat. No.   Influenza B virus Nucleoprotein antibody [GT382] Ms mAb WB, ICC/IF, ELISA, Sandwich ELISA GTX629883   Avian Influenza A virus H5N1 HA (Hemagglutinin) antibody [1D10] Ms mAb WB, ICC/IF GTX632358   Avian Influenza A virus H5N1 NA (Neuraminidase) antibody Rb pAb WB GTX127984   Avian Influenza A virus H5N3 HA (Hemagglutinin) antibody Rb pAb WB GTX127299 Avian Influenza A virus H5N8 HA (Hemagglutinin) antibody [HL1550] Rb mAb WB, IHC-P (cell pellet) GTX637025 Avian Influenza A virus H7N7 HA (Hemagglutinin) antibody Rb pAb WB GTX127303   Avian Influenza A virus H7N7 HA (Hemagglutinin) antibody  Rb pAb WB GTX127302    Avian Influenza A virus H9N2 HA (Hemagglutinin) antibody Rb pAb WB GTX127307   Avian Influenza A virus H9N2 HA (Hemagglutinin) antibody  Rb pAb WB GTX127306   Avian Influenza A virus H9N2 HA (Hemagglutinin) antibody Rb pAb WB GTX127305    Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody Rb pAb WB  GTX135762   Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1342] Rb mAb WB, ICC/IF, IHC-P (cell pellet)  GTX636762 Swine Influenza A virus G4 EA H1N1 HA (Hemagglutinin) antibody [HL1343] Rb mAb WB, ICC/IF, IHC-P (cell pellet) GTX636763