APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| Recommended starting dilution is 1:1000. |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
Based on internal testing result, this antibody does not cross react with human YWHAZ, human YWHAB, human YWHAE, human YWHAH, human SFN, and human YWHAQ.
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT4B9 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human 14-3-5 gamma protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma , PPP1R170 , YWHAG , tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein gamma , 14-3-3 gamma , 1433GAMMA
Cellular Localization
Cytoplasm
Background
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/templates/default/js/lightbox/images/loading.gif "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")
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GTX57599 WB Image
WB analysis of various samples using GTX57599 14-3-3 gamma antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : NIH-3T3 whole cell lysate
Loading : 40 μg
Dilution : 1:1000
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GTX57599 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57599 14-3-3 gamma antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100
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GTX57599 WB Image
WB analysis of various samples using GTX57599 14-3-3 gamma antibody.
Lane 1 : Recombinant Human YWHAZ protein
Lane 2 : Recombinant Human YWHAB protein
Lane 3 : Recombinant Human YWHAE protein
Lane 4 : Recombinant Human YWHAH protein
Lane 5 : Recombinant Human YWHAG protein
Lane 6 : Recombinant Human SFN protein
Lane 7 : Recombinant Human YWHAQ protein
Loading : 50 ng
Dilution : 1:1000
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GTX57599 WB Image
WB analysis of various samples using GTX57599 14-3-3 gamma antibody.
Samples : HeLa whole whole cell lysate and Mouse brain tissue lysate
Loading : 40 μg
Dilution : 1:1000
|
REFERENCE
There are currently no references for 14-3-3 gamma antibody [AT4B9] (GTX57599). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for 14-3-3 gamma antibody [AT4B9] (GTX57599). Be the first to share your experience with this product.
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/upload/website/prouct_img/small/GTX57599/GTX57599_20200204_WB3_w_23061123_986.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57599](/upload/website/prouct_img/small/GTX57599/GTX57599_20200204_IF_w_23061123_707.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/upload/website/prouct_img/small/GTX57599/GTX57599_20200204_WB2_w_23061123_660.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/upload/website/prouct_img/small/GTX57599/GTX57599_20200204_WB1_w_23061123_627.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/upload/website/prouct_img/normal/GTX57599/GTX57599_20200204_WB3_w_23061123_986.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57599](/upload/website/prouct_img/normal/GTX57599/GTX57599_20200204_IF_w_23061123_707.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/upload/website/prouct_img/normal/GTX57599/GTX57599_20200204_WB2_w_23061123_660.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")
![Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599](/upload/website/prouct_img/normal/GTX57599/GTX57599_20200204_WB1_w_23061123_627.webp "Anti-14-3-3 gamma antibody [AT4B9] used in Western Blot (WB). GTX57599")