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14-3-3 gamma antibody

Comparison
Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
Anti-14-3-3 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX113298
Anti-14-3-3 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113298
Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298
Anti-14-3-3 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX113298

Cat. No. GTX113298

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all 14-3-3 gamma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain , K562 , THP-1 , HL-60

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.05 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 gamma. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036124

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma , 14-3-3GAMMA , EIEE56 , PPP1R170

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298

GTX113298 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000 and competitor's antibody (SC-731) diluted by 1:200. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-14-3-3 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX113298

GTX113298 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using 14-3-3 gamma(GTX113298) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-14-3-3 gamma antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113298

GTX113298 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using 14-3-3 gamma(GTX113298) antibody at 1:200 dilution.

Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298

GTX113298 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 14-3-3 gamma antibody (GTX113298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 gamma antibody used in Western Blot (WB). GTX113298

GTX113298 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113298 diluted at 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 gamma antibody used in IHC (Paraffin sections) (IHC-P). GTX113298

GTX113298 IHC-P Image

14-3-3 gamma antibody detects 14-3-3 gamma protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
14-3-3 gamma stained by 14-3-3 gamma antibody (GTX113298) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Iwahori S et al. J Biol Chem 2022; 298 (11) : 102513 Serine 13 of the human cytomegalovirus viral cyclin-dependent kinase UL97 is required for regulatory protein 14-3-3 binding and UL97 stability.
Application : WB, ICC/IF
Reactivity : Human
Sundararaman B et al. Mol Cell 2016; 61 (6) : 903-13 Resources for the Comprehensive Discovery of Functional RNA Elements.
Application : IP
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169