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14-3-3 sigma antibody [1.T.28]

Anti-14-3-3 sigma antibody [1.T.28] used in Western Blot (WB). GTX14122

Cat. No. GTX14122

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1.T.28

Isotype

 IgG1

Application

 WB, IP

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all 14-3-3 sigma products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml (EGF-stimulated A431 cell)
IP Assay dependent
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Product Note

Recognizes human 14-3-3 sigma, Mr 30kD. Does not crossreact with other 14-3-3 isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.6

Preservative

0.07% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

GST fusion protein corresponding to full-length human 14-3-3 sigma

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SFN , YWHAS , stratifin , 14-3-3 sigma

Cellular Localization

Cytoplasm,Nucleus,Secreted

Background

This gene encodes a cell cycle checkpoint protein. The encoded protein binds to translation and initiation factors and functions as a regulator of mitotic translation. In response to DNA damage this protein plays a role in preventing DNA errors during mitosis. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-14-3-3 sigma antibody [1.T.28] used in Western Blot (WB). GTX14122

GTX14122 WB Image

WB analysis of A431 cell lysate using GTX14122 14-3-3 sigma antibody [1.T.28].
Dilution : 0.5μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399