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14-3-3 theta antibody

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199
Anti-14-3-3 theta antibody used in IHC (Paraffin sections) (IHC-P). GTX102199
Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

Cat. No. GTX102199

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:30000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

Mouse brain , 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , HeLa

Predict Reactivity

Rat, Rabbit, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.84 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 14-3-3 theta. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1952636

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein theta , 14-3-3 , 1C5 , HS1

Cellular Localization

Cytoplasm

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence which is polymorphic, however, there is no correlation between the repeat number and the disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Sample(30 μg whole cell lysate)
A: 293T
B: A431 (GTX27909)
C: H1299
D: HeLa S3 (GTX14654)
E: HepG2 (GTX27900)
F: MOLT4 (GTX27912)
G: Raji (GTX27908)
12% SDS PAGE
GTX102199 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with 14-3-3 theta antibody (GTX102199) diluted at 1:1000 and competitor's antibody (SC-732) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Sample (20 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX102199 diluted at 1:30000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-14-3-3 theta antibody used in IHC (Paraffin sections) (IHC-P). GTX102199

GTX102199 IHC-P Image

14-3-3 theta antibody detects 14-3-3 theta protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
14-3-3 theta antibody (GTX102199) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-14-3-3 theta antibody used in Western Blot (WB). GTX102199

GTX102199 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
B: zebrafish heart
12% SDS PAGE
GTX102199 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169