14-3-3 zeta (phospho Ser58) antibody

Cat. No. GTX79155

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse
Package	100 μg ($399)

See all 14-3-3 zeta products

Datasheet

View product citations for antibody GTX79155 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500 - 1:1000
IHC-P	1:50 - 1:100

Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human 14-3-3 ζ around the phosphorylation site of serine 58 (R-S-Sp-W-R).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11172241

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

HEL4,HELS3,HELS93,KCIP1,YWHAD,YWHAZ,tyrosine 3monooxygenase/tryptophan 5monooxygenase activation protein zeta,tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta,14-3-3 zeta,1433zeta

Cellular Localization

Cytoplasm,Melanosome

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

Database

Gene ID: 7534 YWHAZ
UniProt: P63104 YWHAZ

Research Area

DATA IMAGES

GTX79155 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79155 14-3-3 zeta (phospho Ser58) antibody.

GTX79155 WB Image

WB analysis of NIH/3T3 cells untreated or treated with TNF-α (20ng/ml, 5min) lysates using GTX79155 14-3-3 zeta (phospho Ser58) antibody (Lane 3 and 4) and 14-3-3 zeta antibody (Lane 1 and 2).

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

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