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15-PGDH antibody

Orthogonal
Anti-15-PGDH antibody used in Western Blot (WB). GTX113740
Anti-15-PGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113740
Anti-15-PGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX113740

Cat. No. GTX113740

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all 15-PGDH products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Rat, Bovine, Dog, Guinea pig, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human 15-PGDH. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10731270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

15-hydroxyprostaglandin dehydrogenase , 15-PGDH , PGDH , PGDH1 , PHOAR1 , SDR36C1

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-15-PGDH antibody used in Western Blot (WB). GTX113740

GTX113740 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with 15-PGDH antibody (GTX113740) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-15-PGDH antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113740

GTX113740 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using 15-PGDH(GTX113740) antibody at 1:500 dilution.

Anti-15-PGDH antibody used in IHC (Paraffin sections) (IHC-P). GTX113740

GTX113740 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using 15-PGDH(GTX113740) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
Application : WB
Reactivity : Human
Fredman G et al. Nat Commun 2016; 7 : 12859 An imbalance between specialized pro-resolving lipid mediators and pro-inflammatory leukotrienes promotes instability of atherosclerotic plaques.
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169