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5-Carboxylcytosine / 5-caC antibody

Anti-5-Carboxylcytosine / 5-caC antibody used in ChIP assay (ChIP assay). GTX60801
Anti-5-Carboxylcytosine / 5-caC antibody used in Dot blot (Dot). GTX60801
Anti-5-Carboxylcytosine / 5-caC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60801

Cat. No. GTX60801

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 ICC/IF, IP, Dot, ChIP assay

Reactivity

 Species independent
Package
50 μg ($399)
See all 5-Carboxylcytosine / 5-caC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:500
IP 1-5 μg
Dot 1:500 - 1:1,000
ChIP assay Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

5-Carboxylcytosine (5ca-CMP monophosphate) conjugated to BSA.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-5-Carboxylcytosine / 5-caC antibody used in ChIP assay (ChIP assay). GTX60801

GTX60801 ChIP assay Image

ChIP analysis of 2 μg of J1 ES genomic DNA spiked with 1 pg of a control DNA fragment (approximately 700 bp from the RFP (Ring finger protein) gene) containing different cytosine modifications using GTX60801 5-Carboxylcytosine / 5-caC antibody. The mC and hmC control DNA was generated by PCR with the corresponding nucleotide. The caC control fragment was obtained by in vitro methylation using M.SssI methyltransferase followed by oxidation with purified Tet2. The IP'd DNA was subsequently anaysed by qPCR using primers specific for the control DNA fragments and for GAPDH, used as a negative control.

Anti-5-Carboxylcytosine / 5-caC antibody used in Dot blot (Dot). GTX60801

GTX60801 Dot Image

Dot blot analysis of 25 and 125 ng of the synthetic oligonucleotides containing different modified C-bases (indicated in red) using GTX60801 5-Carboxylcytosine / 5-caC antibody.
Dilution : 1:1,000

Anti-5-Carboxylcytosine / 5-caC antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60801

GTX60801 ICC/IF Image

ICC/IF analysis of 293T cells overexpressing either the mouse FLAG-tagged wild-type Tet1 (Tet1 CD) or the catalytically inactive FLAG-tagged C-terminal domain of Tet1 (Tet1 mCD) using GTX60801 5-Carboxylcytosine / 5-caC antibody.
Dilution : 1:500

REFERENCE

Application Reference

Yan JB et al. Mol Ther Oncolytics 2020; 18 : 282-294
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REVIEW

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Package List Price ($)
$ 399