5-HT2A receptor antibody

Cat. No. GTX03473

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($399)

See all 5-HT2A receptor products

Datasheet

View product citations for antibody GTX03473 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
ICC/IF	10μg/ml
IHC-P	2μg/ml
FACS	10ug/ml

Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Product Note

This antibody is expected to recognize both reported isoforms (NP_000612.1; NP_001159419.1).

PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.5% BSA

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-QRSIHREPGSYTGR (aa296-309), from the internal region of the protein sequence according to NP_000612.1; NP_001159419.1.

Purification

Ammonium sulphate precipitation followed by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-hydroxytryptamine receptor 2A , 5-HT2A , HTR2

Cellular Localization

Cell membrane , Cell projection, dendrite , Cytoplasmic vesicle

Background

This gene encodes one of the receptors for serotonin, a neurotransmitter with many roles. Mutations in this gene are associated with susceptibility to schizophrenia and obsessive-compulsive disorder, and are also associated with response to the antidepressant citalopram in patients with major depressive disorder (MDD). MDD patients who also have a mutation in intron 2 of this gene show a significantly reduced response to citalopram as this antidepressant downregulates expression of this gene. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]

Database

Gene ID: 3356 HTR2A
UniProt: P28223 HTR2A

Research Area

DATA IMAGES

GTX03473 IHC-P Image

IHC-P analysis of human cerebellum tissue using GTX03473 5-HT2A receptor antibody.
Antigen retrieval : Microwaved antigen retrieval with citrate buffer pH 6.
Dilution : 2μg/ml

GTX03473 FACS Image

FACS analysis of PFA-fixed A549 cells using GTX03473 5-HT2A receptor antibody.
Blue : Primary antibody
Black : Isotype control
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

GTX03473 ICC/IF Image

ICC/IF analysis of PFA-fixed A549 cells using GTX03473 5-HT2A receptor antibody.
Green : Primary antibody
Blue : DAPI
Negative control (-ve control) : Unimmunized goat IgG followed by secondary antibody
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

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REVIEW

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Package

List Price ($)

100 μg

$ 399

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