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5-HT2C receptor antibody

Orthogonal
Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329
Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329
Anti-5-HT2C receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100329
Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329
Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329

Cat. No. GTX100329

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all 5-HT2C receptor products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

rat brain , mouse placenta

Predict Reactivity

Dog, Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human 5-HT2C Receptor. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950531

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-hydroxytryptamine receptor 2C , 5-HT1C , 5-HT2C , 5-HTR2C , 5HTR2C , HTR1C

Cellular Localization

Cell membrane

Background

Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in humans have reported abnormalities in patterns of 5-HT2C editing in depressed suicide victims. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329

GTX100329 WB Image

Orthogonal

Unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329

GTX100329 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT2C receptor antibody used in IHC (Paraffin sections) (IHC-P). GTX100329

GTX100329 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human prostate, using 5HT2C Receptor(GTX100329) antibody(10 μg/ml).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329

GTX100329 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-5-HT2C receptor antibody used in Western Blot (WB). GTX100329

GTX100329 WB Image

Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with 5-HT2C receptor antibody (GTX100329) diluted at 1:1000.

REFERENCE

Application Reference

2021; : e12771 Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation.
Wang M et al. J Neuroinflammation 2021; 18 (1) : 146 Thymosin β4 reverses phenotypic polarization of glial cells and cognitive impairment via negative regulation of NF-κB signaling axis in APP/PS1 mice.
Application : WB, IHC-Fr
Reactivity : Mouse
Submit a Reference

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Currently in production