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5-Hydroxymethylcytosine / 5-hmC antibody [GT513]

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT513] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629701
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT513] used in Dot blot (Dot). GTX629701
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT513] used in Dot blot (Dot). GTX629701

Cat. No. GTX629701

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT513

Isotype

 IgG2b

Application

 Dot, MeDIP

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 5-Hydroxymethylcytosine / 5-hmC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
Dot Assay dependent
MeDIP Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to 5-Hydroxymethylcytosine / 5-hmC

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888136

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5hmc Antibody , 5-hmc Antibody , 5-HydroxymethylCytidine Antibody

Research Area

DATA IMAGES

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT513] used in Methylated DNA Immunoprecipitation (MeDIP). GTX629701

GTX629701 MeDIP Image

GTX629701 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody [GT513] (GTX629701) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT513] used in Dot blot (Dot). GTX629701

GTX629701 Dot Image

GTX629701 5-hydroxymethylcytosine antibody [GT513] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629701) at a dilution of 1:500. A: Unmethylated DNA fragment B: DNA fragment containing 5-methylcytosine C: DNA fragment containing 5-hydroxymethylcytosine D: DNA fragment containing 5-formylcytosine E: DNA fragment containing 5-carboxylcytosine

Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT513] used in Dot blot (Dot). GTX629701

GTX629701 Dot Image

Dotblot analysis of anti- 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] with DNA samples
DNA samples (50 to 500 ng as indicated) were spotted onto positively charged nylon membrane and blotted with 5-Hydroxymethylcytosine / 5-hmC antibody [GT513] (GTX629701) at a dilution of 1:500.
A: Genomic DNA derived from 293T cells.
B: Genomic DNA derived from human TET-1 transfected 293T cells.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations