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53BP1 antibody

Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64370
Anti-53BP1 antibody used in Western Blot (WB). GTX64370
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370
Anti-53BP1 antibody used in Western Blot (WB). GTX64370
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370
Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64370
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370
Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

Cat. No. GTX64370

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all 53BP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

214 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1763-1977 of human TP53BP1 (NP_001135452.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

Cellular Localization

Nucleus

Background

This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64370

GTX64370 ICC/IF Image

ICC/IF analysis of GFP-RNF168 transgenic U2OS cells using GTX64370 53BP1 antibody.
Green : GFP-RNF168 fusion protein expression for DNA damage marker
Blue : DAPI

Anti-53BP1 antibody used in Western Blot (WB). GTX64370

GTX64370 WB Image

WB analysis of extracts from normal (control) and 53BP1 knockout (KO) 293T cells using GTX64370 53BP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

GTX64370 IHC-P Image

IHC-P analysis of human brain tissue using GTX64370 53BP1 antibody.
Dilution : 1:100

Anti-53BP1 antibody used in Western Blot (WB). GTX64370

GTX64370 WB Image

WB analysis of A431 cell lysate using GTX64370 53BP1 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

GTX64370 IHC-P Image

IHC-P analysis of human brain tissue using GTX64370 53BP1 antibody.
Dilution : 1:100

Anti-53BP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX64370

GTX64370 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX64370 53BP1 antibody.
Blue : DAPI

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

GTX64370 IHC-P Image

IHC-P analysis of human esophageal tissue using GTX64370 53BP1 antibody.
Dilution : 1:100

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

GTX64370 IHC-P Image

IHC-P analysis of human brain tissue using GTX64370 53BP1 antibody.
Dilution : 1:100

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

GTX64370 IHC-P Image

IHC-P analysis of human esophageal tissue using GTX64370 53BP1 antibody.
Dilution : 1:100

Anti-53BP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX64370

GTX64370 IHC-P Image

IHC-P analysis of human brain tissue using GTX64370 53BP1 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Li G et al. J Cell Sci 2021; 134 (2) Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage.
Application : WB, IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399