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6X His tag antibody (HRP)

Anti-6X His tag antibody (HRP) used in Western Blot (WB). GTX115045-01

Cat. No. GTX115045-01

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Species independent
Package
100 μl ($399),
25 μl ($169)
See all 6X His tag products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Positive Control

His-tagged recombinant protein

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 1%BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Rabbits were immunized with HHHHHH conjugated with KLH. After multiple immunizations in Freunds adjuvant serum was collected. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_11170588

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

Research Area

DATA IMAGES

Anti-6X His tag antibody (HRP) used in Western Blot (WB). GTX115045-01

GTX115045-01 WB Image

Western blot analysis of extracts from untransfected control cells and transfected cells overexpressing His-tagged protein (~27 kDa), using 6X 6X His tag antibody conjugated with HRP.

A: 10ug E.coli Lysate harbouring His-tagged
recombinant protein (N-terminal linked),
non-induced (10 ug)

B: 10ug E.coli Lysate harbouring His-tagged
recombinant protein (N-terminal linked),
induced (10 ug)

12% SDS PAGE

GTX115045-01 diluted at 1:500

REFERENCE

Application Reference

Ebert S et al. Cells 2023; 12 (14) Tissue Inhibitor of Metalloproteinases-1 Interacts with CD74 to Promote AKT Signaling, Monocyte Recruitment Responses, and Vascular Smooth Muscle Cell Proliferation.
Submit a Reference

REVIEW

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SDS
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169