ABIN3 antibody

Cat. No. GTX85255

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, IHC-P, ELISA
Reactivity	Human, Mouse
Package	100 μg ($399)

See all ABIN3 products

Datasheet
Document

View product citations for antibody GTX85255 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1 μg/mL
IHC-P	5 μg/mL
ELISA	Assay dependent

Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

Human Spleen Tissue Lysate(GTX27921)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

ABIN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ABIN3.The immunogen is located within the last 50 amino acids of ABIN3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TNFAIP3 interacting protein 3 , ABIN-3 , LIND

Background

The nuclear factor NF-kB plays key roles in development and immunity. ABIN3 (A20-binding inhibitor of NF-kB activation 3), also known as TNFAIP3-interacting protein 3 (TNIP3), is a novel negative feedback regulator of LPS-induced NF-kB activation. ABIN3 is a 39 kDa protein that negatively regulates NF-kB activation in response to TNF and LPS. ABIN3 is highly expressed in brain, thymus, lymph node, lung and fetal liver, with low expression in kidney, bone marrow. Through its interaction with A20, ABIN3 interferes with TRAF2-mediated transactivation signals and NF-kB inhibition is mediated by the ABIN-homology domain 2. ABIN3 has been found to be induced by Listeria infection and can be slightly downregulated by dexamethasone. Enhanced expression of ABIN3 in monocytes is associated with sepsis. Thus, ABIN3 is an IL-10-induced gene product capable of attenuating NF-kB in human macrophages yet is inoperative in mice and represents a basis for species-specific differences in IL-10 actions. At least four isoforms of ABIN3 are known to exist.

Database

Gene ID: 79931 TNIP3
UniProt: Q96KP6 TNIP3

Research Area

DATA IMAGES

GTX85255 IHC-P Image

IHC-P analysis of mouse spleen tissue using GTX85255 ABIN3 antibody.
Working concentration : 5 μg/ml

GTX85255 WB Image

WB analysis of human spleen tissue lysate in (A) the absence and (B) the presence of blocking peptide using GTX85255 ABIN3 antibody.
Working concentration : 1 μg/ml

REFERENCE

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty