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ACAT2 antibody [AT55D5]

Anti-ACAT2 antibody [AT55D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57610
Anti-ACAT2 antibody [AT55D5] used in Western Blot (WB). GTX57610
Anti-ACAT2 antibody [AT55D5] used in Western Blot (WB). GTX57610
Anti-ACAT2 antibody [AT55D5] used in Flow cytometry (FACS). GTX57610

Cat. No. GTX57610

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 AT55D5

Isotype

 IgG2b

Application

 WB, ICC/IF, FACS

Reactivity

 Human
Package
100 μl ($399)
See all ACAT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Recommended starting dilution is 1:1000.
ICC/IF Assay dependent
FACS Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT55D5 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human ACAT4 protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acetyl-CoA acetyltransferase 2 , ACAT2 , acetylCoA acetyltransferase 2

Cellular Localization

Cytoplasm

Background

The product of this gene is an enzyme involved in lipid metabolism, and it encodes cytosolic acetoacetyl-CoA thiolase. This gene shows complementary overlapping with the 3-prime region of the TCP1 gene in both mouse and human. These genes are encoded on opposite strands of DNA, as well as in opposite transcriptional orientation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2014]

Database

  • Gene ID: 39 ACAT2
  • UniProt: Q9BWD1 ACAT2

Research Area

DATA IMAGES

Anti-ACAT2 antibody [AT55D5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57610

GTX57610 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX57610 ACAT2 antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-ACAT2 antibody [AT55D5] used in Western Blot (WB). GTX57610

GTX57610 WB Image

WB analysis of various samples using GTX57610 ACAT2 antibody.
Lane 1 : 293T cell lysate
Lane 2 : ACAT2 transfected 293T cell lysate
Loading : 5 μg
Dilution : 1:1000

Anti-ACAT2 antibody [AT55D5] used in Western Blot (WB). GTX57610

GTX57610 WB Image

WB analysis of various samples using GTX57610 ACAT2 antibody.
Lane 1 : HepG2 whole cell lysate
Lane 2 : Ramos whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : 293T whole cell lysate
Lane 5 : HL-60 whole cell lysate
Lane 6 : SW480 whole cell lysate
Loading : 40 μg
Dilution : 1:1000

Anti-ACAT2 antibody [AT55D5] used in Flow cytometry (FACS). GTX57610

GTX57610 FACS Image

FACS analysis of PC-3 cells using GTX57610 ACAT2 antibody.
Cell Number: 1 x 10⁶ cells
Upper panel : negative control, Lower panel: Primary antibody
Antibody amount: 2-5 μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399