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ADAM1B antibody [#158]

Orthogonal
Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690
Anti-ADAM1B antibody [#158] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00690
Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690

Cat. No. GTX00690

Reference 3

Host

 Rat

Clonality

 Monoclonal

Clone Name

 #158

Isotype

 IgG2a

Application

 WB, ICC/IF

Reactivity

 Mouse
Package
100 μg ($399)
See all ADAM1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100-1:500
ICC/IF 1:300
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Product Note

This antibody showed no reactivity with ADAM1A (confirmed by WB using recombinant ADAM1A and ADAM1B proteins).

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse sperm

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

a disintegrin and metallopeptidase domain 1b , Adam1 , Ftna

Cellular Localization

Membrane

Database

DATA IMAGES

Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690

GTX00690 WB Image

Orthogonal

WB analysis of mouse testis lysates collected from different ages using GTX00690 ADAM1B antibody [#158].
ADAM1B appeared as a faint signal at 3 weeks of age.

Anti-ADAM1B antibody [#158] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00690

GTX00690 ICC/IF Image

ICC/IF analysis of fresh mouse sperms using GTX00690 ADAM1B antibody [#158].
Dilution : 1:300
Fixation : 4% PFA

Anti-ADAM1B antibody [#158] used in Western Blot (WB). GTX00690

GTX00690 WB Image

Orthogonal

WB analysis of various mouse tissue samples using GTX00690 ADAM1B antibody [#158]. The Adam1B synthesized in testis (120 kDa) is proteolytically processed into smaller form (60 kDa) in sperm during the epididymal maturation of the spermatozoa. By western blotting, ADAM1B is detected in testis extract at 120 kDa position, which is due to glycosylation and larger than predicted size of 89 kDa from the sequence.
This protien is testis-specific.
Dilution : 1:100
This protien is testis-specific.

REFERENCE

Clone Reference

Yamaguchi R et al. Mol Biol Cell. 2012; 23 (14) Mice expressing aberrant sperm-specific protein PMIS2 produce normal-looking but fertilization-incompetent spermatozoa.
Application : WB
Reactivity : Mouse
Tokuhiro K et al. Proc Natl Acad Sci U S A. 2012; 109 (10) Protein disulfide isomerase homolog PDILT is required for quality control of sperm membrane protein ADAM3 and male fertility [corrected].
Application : WB
Reactivity : Mouse
Ikawa M et al. J Biol Chem. 2011; 286 (7):5639-46 Calsperin is a testis-specific chaperone required for sperm fertility.
Application : WB, ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399