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ADAM1B antibody [#57]

Orthogonal
Anti-ADAM1B antibody [#57] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00691
Anti-ADAM1B antibody [#57] used in IHC (Paraffin sections) (IHC-P). GTX00691

Cat. No. GTX00691

Reference 1

Host

 Rat

Clonality

 Monoclonal

Clone Name

 #57

Isotype

 IgG2a

Application

 ICC/IF, IHC-P

Reactivity

 Mouse
Package
100 μg ($399)
See all ADAM1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100-1:300
IHC-P 1:100-1:300
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Mouse sperm

Purification

Purified IgG

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

a disintegrin and metallopeptidase domain 1b , Adam1 , Ftna

Cellular Localization

Membrane

Database

DATA IMAGES

Anti-ADAM1B antibody [#57] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00691

GTX00691 ICC/IF Image

Orthogonal

ICC/IF analysis of a representative time-lapse view of sperm-egg fusion using GTX00691 ADAM1B antibody [#57].
The initiation of fusion (time 0) was detected by transfer of Hoechst 33342 dye to sperm in the same area (indicated by arrowheads). The diffusion of membrane in the postacrosomal area identified by ADAM1B (green) started about 60 s after the initiation of sperm-egg fusion and was accompanied by expansion of the Hoechst 33342 staining area toward the posterior head.

Anti-ADAM1B antibody [#57] used in IHC (Paraffin sections) (IHC-P). GTX00691

GTX00691 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00691 ADAM1B antibody [#57].
Dilution : 1:100

REFERENCE

Clone Reference

Satouh Y et al. J Cell Sci. 2012; Visualization of the moment of mouse sperm-egg fusion and dynamic localization of IZUMO1.
Application : ICC/IF
Reactivity : Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Package List Price ($)
$ 399