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ADCY3 antibody

Anti-ADCY3 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX04114
Anti-ADCY3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04114
Anti-ADCY3 antibody used in Flow cytometry (FACS). GTX04114
Anti-ADCY3 antibody used in Western Blot (WB). GTX04114

Cat. No. GTX04114

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, FACS, IHC (Free Floating)

Reactivity

 Human, Mouse, Rat
Package
50 μl ($669)
See all ADCY3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:50
FACS Assay dependent
IHC (Free Floating) 1:50-1:200
Not tested in other applications.

Calculated MW

129 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 1% BSA

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.85 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide (C)KHVADEMLKDMKKDE, corresponding to amino acid residues 285-299 of rat ADCY3 (Accession P21932, 3rd extracellular loop).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

adenylate cyclase 3

Cellular Localization

Cell membrane , Golgi apparatus , Cell projection, cilium , Cytoplasm

Background

enzyme that catalyzes the formation of the secondary messenger cyclic adenosine monophosphate [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-ADCY3 antibody used in IHC (Free Floating) (IHC (Free Floating)). GTX04114

GTX04114 IHC (Free Floating) Image

IHC (Free Floating) analysis of mouse hippocampus tissue using GTX04114 ADCY3 antibody.
Panel A : ADCY3 staining (red)
Panel B : NeuN staining (green) as a neuronal marker on the same section.
Panel C : Merge of A and B demonstrates "primary cilia" - thin rod-like extensions from neurons in the pyramidal layer of the hippocampus.
Dilution : 1:400

Anti-ADCY3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04114

GTX04114 ICC/IF Image

Live cell imaging analysis of U-87 MG cells using GTX04114 ADCY3 antibody.
Panel A : Primary antibody
Panel B : Merge of A with live view of the cells
Dilution : 1:50

Anti-ADCY3 antibody used in Flow cytometry (FACS). GTX04114

GTX04114 FACS Image

FACS analysis of MEG-01 cells using GTX04114 ADCY3 antibody.
Black : Unlabel samples
Red : Isotype control
Green : Primary antibody
Antibody amount : 2.5 μg

Anti-ADCY3 antibody used in Western Blot (WB). GTX04114

GTX04114 WB Image

WB analysis of various tissue lysates using GTX04114 ADCY3 antibody(Lane 1 - 3) preincubated with blocking peptide (Lane 4 - 6).
Lane 1, 4 : Rat lung
Lane 2, 5 : Rat brain
Lane 3, 6 : Rat hippocampus
Dilution : 1:200

REFERENCE

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REVIEW

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Package List Price ($)
$ 669