APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:100 - 1:500 |
| 1:100 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol
Preservative
0.01% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of AKAP13. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
A-kinase anchoring protein 13 , AKAP-13 , AKAP-Lbc , ARHGEF13 , BRX , HA-3 , Ht31 , LBC , PRKA13 , PROTO-LB , PROTO-LBC , c-lbc , p47
Cellular Localization
Cytoplasm, cytosol,Cytoplasm,Nucleus,Membrane
Background
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms containing c-terminal dbl oncogene homology (DH) and pleckstrin homology (PH) domains. The DH domain is associated with guanine nucleotide exchange activation for the Rho/Rac family of small GTP binding proteins, resulting in the conversion of the inactive GTPase to the active form capable of transducing signals. The PH domain has multiple functions. Therefore, these isoforms function as scaffolding proteins to coordinate a Rho signaling pathway, function as protein kinase A-anchoring proteins and, in addition, enhance ligand-dependent activity of estrogen receptors alpha and beta. [provided by RefSeq, Jul 2012]
Database
Research Area
DATA IMAGES
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GTX54888 ICC/IF Image
ICC/IF analysis of formalin-fixed HeLa cells using GTX54888 AKAP13 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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GTX54888 WB Image
WB analysis of HeLa (A) whole cell lysates using GTX54888 AKAP13 antibody.
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GTX54888 IHC-P Image
IHC-P analysis of formalin fixed human brain tissue section using GTX54888 AKAP13 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)
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REFERENCE
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REVIEW
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