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AKR1A1 antibody [8H2]

KO/KD Overexpression
Anti-AKR1A1 antibody [8H2] used in Flow cytometry (FACS). GTX84915
Anti-AKR1A1 antibody [8H2] used in IHC (Paraffin sections) (IHC-P). GTX84915
Anti-AKR1A1 antibody [8H2] used in Western Blot (WB). GTX84915
Anti-AKR1A1 antibody [8H2] used in IHC (Paraffin sections) (IHC-P). GTX84915
Anti-AKR1A1 antibody [8H2] used in Flow cytometry (FACS). GTX84915
Anti-AKR1A1 antibody [8H2] used in Western Blot (WB). GTX84915

Cat. No. GTX84915

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8H2

Isotype

 IgG2b

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all AKR1A1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:2000
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human AKR1A1 (NP_006057) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member A1 , ALDR1 , ALR , ARM , DD3 , HEL-S-6

Cellular Localization

Cytoplasm, cytosol,Apical cell membrane

Background

Catalyzes the NADPH-dependent reduction of a variety of aromatic and aliphatic aldehydes to their corresponding alcohols. Catalyzes the reduction of mevaldate to mevalonic acid and of glyceraldehyde to glycerol. Has broad substrate specificity. In vitro substrates include succinic semialdehyde, 4-nitrobenzaldehyde, 1,2-naphthoquinone, methylglyoxal, and D-glucuronic acid. Plays a role in the activation of procarcinogens, such as polycyclic aromatic hydrocarbon trans-dihydrodiols, and in the metabolism of various xenobiotics and drugs, including the anthracyclines doxorubicin (DOX) and daunorubicin (DAUN).

Database

Research Area

DATA IMAGES

Anti-AKR1A1 antibody [8H2] used in Flow cytometry (FACS). GTX84915

GTX84915 FACS Image

FACS analysis of Jurkat cells using GTX84915 AKR1A1 antibody [8H2].
Red : Primary antibody
Blue : Negative control antibody

Anti-AKR1A1 antibody [8H2] used in IHC (Paraffin sections) (IHC-P). GTX84915

GTX84915 IHC-P Image

IHC-P analysis of liver tissue using GTX84915 AKR1A1 antibody [8H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-AKR1A1 antibody [8H2] used in Western Blot (WB). GTX84915

GTX84915 WB Image

Overexpression

WB analysis of HEK293T cells transfected with AKR1A1 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84915 AKR1A1 antibody [8H2].
Loading : 5 ug per lane

Anti-AKR1A1 antibody [8H2] used in IHC (Paraffin sections) (IHC-P). GTX84915

GTX84915 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84915 AKR1A1 antibody [8H2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-AKR1A1 antibody [8H2] used in Flow cytometry (FACS). GTX84915

GTX84915 FACS Image

FACS analysis of HeLa cells using GTX84915 AKR1A1 antibody [8H2].
Red : Primary antibody
Blue : Negative control antibody

Anti-AKR1A1 antibody [8H2] used in Western Blot (WB). GTX84915

GTX84915 WB Image

KO/KD

WB analysis of wild type and AKR1A1 knock out 293T cell lysate(10μg per lane) using GTX84915 AKR1A1 antibody [8H2].
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519