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AKR1B1 antibody

Anti-AKR1B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54911
Anti-AKR1B1 antibody used in Western Blot (WB). GTX54911
Anti-AKR1B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54911

Cat. No. GTX54911

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all AKR1B1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of AKR1B1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member B , ADR , ALDR1 , ALR2 , AR

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

Database

Research Area

DATA IMAGES

Anti-AKR1B1 antibody used in IHC (Paraffin sections) (IHC-P). GTX54911

GTX54911 IHC-P Image

IHC-P analysis of formalin fixed human colon cancer tissue section using GTX54911 AKR1B1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-AKR1B1 antibody used in Western Blot (WB). GTX54911

GTX54911 WB Image

WB analysis of Jurkat (A), HeLa (B), SP2/0 (C), PC12 (D) whole cell lysates using GTX54911 AKR1B1 antibody.

Anti-AKR1B1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX54911

GTX54911 ICC/IF Image

ICC/IF analysis of formalin-fixed Jurkat cells using GTX54911 AKR1B1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399