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AKR1C1 antibody

KO/KD Orthogonal Overexpression
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Anti-AKR1C1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105620
Anti-AKR1C1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105620

Cat. No. GTX105620

Reference 14
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all AKR1C1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

mouse liver , A549 , AKR1C1-transfected 293T

Predict Reactivity

Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AKR1C1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949621

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aldo-keto reductase family 1 member C1 , 2-ALPHA-HSD , 20-ALPHA-HSD , C9 , DD1 , DD1/DD2 , DDH , DDH1 , H-37 , HAKRC , HBAB , MBAB

Cellular Localization

Cytoplasm

Background

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKR1C1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105620

GTX105620 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using AKR1C1(GTX105620) antibody at 1:200 dilution.

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKR1C1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105620

GTX105620 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft , using AKR1C1(GTX105620) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX105620 diluted at 1:1000

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

AKR1C1 antibody detects AKR1C1 protein by western blot analysis.
A. 50 μg mouse liver lysate/extract
10% SDS-PAGE
AKR1C1 antibody (GTX105620) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKR1C1 antibody (GTX105620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Orthogonal

The data was published in the 2021 in J Cell Mol Med. PMID: 34114342

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Orthogonal

The data was published in the 2021 in J Cell Mol Med. PMID: 34114342

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Orthogonal

The data was published in the 2021 in J Cell Mol Med. PMID: 34114342

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Overexpression

The data was published in the journal Theranostics in 2020. PMID: 32104503

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Overexpression

The data was published in the journal Front Physiol in 2019. PMID: 31333491

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

Overexpression

The data was published in the journal Theranostics in 2020. PMID: 32104503

Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620

GTX105620 WB Image

KO/KD Overexpression

The data was published in the journal Theranostics in 2020. PMID: 32104503

Anti-AKR1C1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105620

GTX105620 ICC/IF Image

The data was published in the journal Theranostics in 2020. PMID: 32104503

Anti-AKR1C1 antibody used in IHC (Paraffin sections) (IHC-P). GTX105620

GTX105620 IHC-P Image

The data was published in the 2021 in J Cell Mol Med. PMID: 34114342

REFERENCE

Application Reference

S Zhen et al. Cell Death Discov 2024; 10 (1) : 131 NEAT1_1 confers gefitinib resistance in lung adenocarcinoma through promoting AKR1C1-mediated ferroptosis defence
Application : WB
Paul M et al. Reprod Sci 2024; 31 (1) : 150-161 Regulation of 20α-Hydroxysteroid Dehydrogenase Expression in Term Pregnant Human Myometrium Ex Vivo.
Paul M et al. Reprod Sci 2023; 30 (8) : 2512-2523 20α-Hydroxysteroid Dehydrogenase Expression in the Human Myometrium at Term and Preterm Birth: Relationships to Fetal Sex and Maternal Body Mass Index.
Lin-Lin Chang et al. Transl Oncol 2022; 20 : 101421 AKR1C1 promotes non-small cell lung cancer proliferation via crosstalk between HIF-1α and metabolic reprogramming.
Application : WB, IHC-P
Nadeem L et al. J Cell Mol Med 2021; 25 (14) : 6773-6785 Pro-inflammatory signals induce 20α-HSD expression in myometrial cells: A key mechanism for local progesterone withdrawal.
Application : WB, ICC/IF
Reactivity : Mouse
Show More +
Submit a Reference

REVIEW

AKR1C1 antibody Cat. No. GTX105620

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Date : Anonymous submitted on 22-Dec-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 10μg
Blocking : IBB buffer (LI-COR), 25°C, 1Hr
Primary Antibody : 1:5000, diluted in IBB buffer, 25°C, 1Hr
Figure Note : 5μL of Dynabeads protein G and 10μg of AKR1C1 antibody (GTX105620) were added to 200μL of PBS containing 0.02% tween-20, and were mixed by inverting for 1 hr. The pellet of HepG2 cell lysate was crosslinked with the antibody-dynabeads complex by mixing. After the pellet was washed with IP buffer containing PBS including 3% triethanolamine, it was diluted in 20% (w/v) SDS as IP samples.IP sample was eluted in 30μl of the buffer and then 20μl was loaded on the SDS-PAGE. That equals to a two-thirds of 500μg original lysate. For the HepG2 input lysate, the loaded amount was 10μg.
Anti-AKR1C1 antibody used in Western Blot (WB). GTX105620
Date : Anonymous submitted on 31-Mar-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : liver cytosol
Amount used : 2 μg
Blocking : Odyssey® Blocking Buffer in PBS, 25°C, 1Hr
Primary Antibody : 1:5000, 25°C, 1Hr
Submit a Review
Application Reference
S Zhen et al. Cell Death Discov 2024; 10 (1):131 NEAT1_1 confers gefitinib resistance in lung adenocarcinoma through promoting AKR1C1-mediated ferroptosis defence
Application : WB
Paul M et al. Reprod Sci 2024; 31 (1):150-161 Regulation of 20α-Hydroxysteroid Dehydrogenase Expression in Term Pregnant Human Myometrium Ex Vivo.
Paul M et al. Reprod Sci 2023; 30 (8):2512-2523 20α-Hydroxysteroid Dehydrogenase Expression in the Human Myometrium at Term and Preterm Birth: Relationships to Fetal Sex and Maternal Body Mass Index.
Lin-Lin Chang et al. Transl Oncol 2022; 20:101421 AKR1C1 promotes non-small cell lung cancer proliferation via crosstalk between HIF-1α and metabolic reprogramming.
Application : WB, IHC-P
Nadeem L et al. J Cell Mol Med 2021; 25 (14):6773-6785 Pro-inflammatory signals induce 20α-HSD expression in myometrial cells: A key mechanism for local progesterone withdrawal.
Application : WB, ICC/IF
Reactivity : Mouse
Marino N et al. NPJ Breast Cancer 2020; 6:50 Upregulation of lipid metabolism genes in the breast prior to cancer diagnosis.
Application : IHC-P
Reactivity : Human
Zhu H et al. Theranostics 2020; 10 (5):2188-2200 The SIRT2-mediated deacetylation of AKR1C1 is required for suppressing its pro-metastasis function in Non-Small Cell Lung Cancer.
Application : WB, ICC/IF
Reactivity : Human
Jiang Y et al. Br J Haematol 2020; (Epub) Upregulation of AKR1C1 in mesenchymal stromal cells promotes the survival of acute myeloid leukaemia cells.
Application : WB
Reactivity : Mouse
Zeng C et al. Front Physiol 2019; 10:833 Liquiritin, as a Natural Inhibitor of AKR1C1, Could Interfere With the Progesterone Metabolism.
Application : WB
Chenming Zeng et al. Front. Physiol. 2019; (Epub) Liquiritin, as a Natural Inhibitor of AKR1C1, Could Interfere With the Progesterone Metabolism.
Application : WB
Reactivity : Human
Zhu H et al. Theranostics 2018; 8 (3):676-692 AKR1C1 Activates STAT3 to Promote the Metastasis of Non-Small Cell Lung Cancer.
ZHU H et al. Theranostics 2018; 8 (3):676-692 AKR1C1 Activates STAT3 to Promote the Metastasis of Non-Small Cell Lung Cancer.
Application : WB, ICC/IF, IHC-P
Reactivity : Human
Gupta R et al. Endocrinology 2017; 158 (8):2572-2584 AgRP-Expressing Adrenal Chromaffin Cells Are Involved in the Sympathetic Response to Fasting.
Nomura M et al. J Clin Bioinforma 2011; 1 (1):23 Preferential expression of potential markers for cancer stem cells in large cell neuroendocrine carcinoma of the lung. An FFPE proteomic study.
Application : IHC
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169