GeneTex
United States (US)

AKT1 antibody [N3C3]

Genetex  AKT1 074S 400X 1in 10000 human esop. cancer 2.2011_19011720_316.jpg
GTX101074_39869_WB_19011506_894.jpg
GTX101074_39869_IHC_3_19011506_682.jpg
GTX101074_39869_IHC_2_19011506_641.jpg
GTX101074_WB_19011506_655.jpg
GTX101074_39869_IFA_19011506_447.jpg

Cat No. GTX101074

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

HA-AKT transfected HeLa cell , A431

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.17 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Akt1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Akt Serine/Threonine Kinase 1,Akt,Cws6,Pkb,Pkb-Alpha,Prkba,Rac,Rac-Alpha,Akt1

Cellular Localization

Cytoplasm , Nucleus , Cell membrane

Background

The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
Genetex  AKT1 074S 400X 1in 10000 human esop. cancer 2.2011_19011720_316.jpg

GTX101074 IHC-P Image

Staining of human esophageal cancer tissue sections using anti-Akt1 antibody (GTX101074)

GTX101074_39869_WB_19011506_894.jpg

GTX101074 WB Image

Sample (20 ug of whole cell lysate)
A: HeLa cell lysate
B: HeLa cell transfect with AKT
7.5% SDS PAGE
GTX101074 diluted at 1:1000

GTX101074_39869_IHC_3_19011506_682.jpg

GTX101074 IHC-P Image

Akt1 antibody detects Akt1 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
Akt1 antibody (GTX101074) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101074_39869_IHC_2_19011506_641.jpg

GTX101074 IHC-P Image

Akt1 antibody detects Akt1 protein at nucleus on human breast cacinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast cacinoma.
Akt1 antibody (GTX101074) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101074_WB_19011506_655.jpg

GTX101074 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
7.5% SDS PAGE
GTX101074 diluted at 1:500

GTX101074_39869_IFA_19011506_447.jpg

GTX101074 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Akt1(GTX101074) antibody at 1:500 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169