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ALIX antibody

Anti-ALIX antibody used in Western Blot (WB). GTX135226

Cat. No. GTX135226

1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ALIX products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.19 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ALIX. The exact sequence is proprietary.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2909834

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

programmed cell death 6 interacting protein , AIP1 , ALIX , DRIP4 , HP95

Cellular Localization

Cytoplasm, cytosol

Background

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Database

Research Area

DATA IMAGES

Anti-ALIX antibody used in Western Blot (WB). GTX135226

GTX135226 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ALIX antibody (GTX135226) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

ALIX antibody Cat. No. GTX135226

Rating
( Average 4 based on 1 users reviews)
Application
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ALIX antibody used in Western Blot (WB). GTX135226
Date : Anonymous submitted on 09-Aug-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : HeLa and Jurkat cells
Amount used : 35μg
Blocking : 5%BSA in TBST, Air temperature°C, 1Hr
Primary Antibody : 1:500, 4°C, 14Hr
Figure Note : The observed M.W. is based on the publication: PMID: 26935291
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Package List Price ($)
$ 399
$ 169