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ALKBH5 antibody

Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525
Anti-ALKBH5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01525
Anti-ALKBH5 antibody used in Flow cytometry (FACS). GTX01525
Anti-ALKBH5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01525
Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525
Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525
Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525
Anti-ALKBH5 antibody used in Flow cytometry (FACS). GTX01525

Cat. No. GTX01525

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all ALKBH5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
IHC-P 1:100
FACS 1:25
Not tested in other applications.

Calculated MW

44 kDa. ( Note )

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 302-330 amino acids from the Central region of human ALKBH5.

Purification

Protein A purified followed by peptide affinity purification

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Cellular Localization

Nucleus speckle

Database

Research Area

DATA IMAGES

Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525

GTX01525 WB Image

WB analysis of 293 whole cell lysate using GTX01525 ALKBH5 antibody.
Dilution : 1:2000
Loading : 20μg per lane

Anti-ALKBH5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01525

GTX01525 IHC-P Image

IHC-P analysis of human testis tissue sections using GTX01525 ALKBH5 antibody.
Dilution : 1:100

Anti-ALKBH5 antibody used in Flow cytometry (FACS). GTX01525

GTX01525 FACS Image

FACS analysis of 2% PFA-fixed U2OS cells using GTX01525 ALKBH5 antibody.
Green : Primary antibody
Blue : Isotype control
Permeabilization : 90% methanol for 10 min
Antibody amount : 1μg/1x10⁶ cells

Anti-ALKBH5 antibody used in IHC (Paraffin sections) (IHC-P). GTX01525

GTX01525 IHC-P Image

IHC-P analysis of human testis tissue sections using GTX01525 ALKBH5 antibody.
Dilution : 1:100

Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525

GTX01525 WB Image

WB analysis of 293 whole cell lysate using GTX01525 ALKBH5 antibody.
Dilution : 1:2000
Loading : 20μg per lane

Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525

GTX01525 WB Image

WB analysis of various sample lysates using GTX01525 ALKBH5 antibody.
Lane 1: HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Dilution : 1:500
Loading : 20 μg per lane

Anti-ALKBH5 antibody used in Western Blot (WB). GTX01525

GTX01525 WB Image

WB analysis of various sample lysates using GTX01525 ALKBH5 antibody.
Lane 1: HeLa whole cell lysate
Lane 2 : MCF-7 whole cell lysate
Dilution : 1:500
Loading : 20 μg per lane

Anti-ALKBH5 antibody used in Flow cytometry (FACS). GTX01525

GTX01525 FACS Image

FACS analysis of 2% PFA-fixed U2OS cells using GTX01525 ALKBH5 antibody.
Green : Primary antibody
Blue : Isotype control
Permeabilization : 90% methanol for 10 min
Antibody amount : 1μg/1x10⁶ cells

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399