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ALOX15 antibody [OTI3G8]

Anti-ALOX15 antibody [OTI3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520
Anti-ALOX15 antibody [OTI3G8] used in Western Blot (WB). GTX04520

Cat. No. GTX04520

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 OTI3G8

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Dog, Monkey
Package
100 μl ($539)
See all ALOX15 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1:100
IHC-P 1:150
Not tested in other applications.

Calculated MW

75 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human ALOX15(NP_001131) produced in HEK293T cell.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

arachidonate 15-lipoxygenase , 12-LOX , 15-LOX , 15-LOX-1 , LOG15

Cellular Localization

Cytoplasm, cytosol , Cell membrane

Background

This gene encodes a member of the lipoxygenase family of proteins. The encoded enzyme acts on various polyunsaturated fatty acid substrates to generate various bioactive lipid mediators such as eicosanoids, hepoxilins, lipoxins, and other molecules. The encoded enzyme and its reaction products have been shown to regulate inflammation and immunity. Multiple pseudogenes of this gene have been identified in the human genome. [provided by RefSeq, Aug 2017]

Database

Research Area

DATA IMAGES

Anti-ALOX15 antibody [OTI3G8] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04520

GTX04520 ICC/IF Image

ICC/IF analysis of COS7 cells with human ALOX15 transfection using GTX04520 ALOX15 antibody [OTI3G8].

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human breast tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human thyroid carcinoma tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human pancreas carcinoma tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human bladder carcinoma tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human prostate carcinoma tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human endometrium tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human endometrium carcinoma tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 10mM citric buffer (pH 6.0) at 100ºC for 10min

Anti-ALOX15 antibody [OTI3G8] used in IHC (Paraffin sections) (IHC-P). GTX04520

GTX04520 IHC-P Image

IHC-P analysis of human lung tissue using GTX04520 ALOX15 antibody [OTI3G8].
Antigen retrieval : 1mM EDTA in 10mM Tris buffer (pH 8.5) at 120ºC for 3 min
Dilution : 1:150

Anti-ALOX15 antibody [OTI3G8] used in Western Blot (WB). GTX04520

GTX04520 WB Image

WB analysis of 293T cells with or without human ALOX15 transfection using GTX04520 ALOX15 antibody [OTI3G8].
Lane 1 : Control vector
Lane 2 : Human ALOX15 transfection
Loading : 5 μg

REFERENCE

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REVIEW

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Package List Price ($)
$ 539