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ALP / PDLIM3 antibody

Anti-ALP / PDLIM3 antibody used in Western Blot (WB). GTX119505
Anti-ALP / PDLIM3 antibody used in Western Blot (WB). GTX119505
Anti-ALP / PDLIM3 antibody used in Western Blot (WB). GTX119505

Cat. No. GTX119505

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ALP / PDLIM3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

293T , A431 , HepG2 , mouse muscle

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ALP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168671

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

PDZ and LIM domain 3 , ALP

Cellular Localization

Cytoplasm , myofibril , sarcomere , Z-disk

Background

The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. This gene is found near a region of chromosome 4 that has been implicated in facioscapulohumeral muscular dystrophy, but this gene does not appear to be involved in the disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ALP / PDLIM3 antibody used in Western Blot (WB). GTX119505

GTX119505 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ALP / PDLIM3 antibody (GTX119505) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALP / PDLIM3 antibody used in Western Blot (WB). GTX119505

GTX119505 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ALP / PDLIM3 antibody (GTX119505) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALP / PDLIM3 antibody used in Western Blot (WB). GTX119505

GTX119505 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HepG2
10% SDS PAGE
GTX119505 diluted at 1:1000

REFERENCE

Application Reference

Lin X et al. Aging Dis 2023; 14 (1) : 170-183 BMF-AS1/BMF Promotes Diabetic Vascular Calcification and Aging both In Vitro and In Vivo.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169