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ALPK1 antibody

Anti-ALPK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01077
Anti-ALPK1 antibody used in Western Blot (WB). GTX01077
Anti-ALPK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX01077

Cat. No. GTX01077

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all ALPK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:1000
ICC/IF 1:100-1:500
IHC-P 1:50-1:200
Not tested in other applications.

Calculated MW

139 kDa. ( Note )

Predict Reactivity

Sheep(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human ALPK1, corresponding to a region within the internal amino acids.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alpha kinase 1 , ALPK1 , LAK , 8430410J10Rik

Background

This gene encodes an alpha kinase. Mice which were homozygous for disrupted copies of this gene exhibited coordination defects (PMID: 21208416). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

Database

Research Area

DATA IMAGES

Anti-ALPK1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01077

GTX01077 ICC/IF Image

ICC/IF analysis of PFA-fixed COLO205 cells using GTX01077 ALPK1 antibody.
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-ALPK1 antibody used in Western Blot (WB). GTX01077

GTX01077 WB Image

WB analysis of HepG2 cell lysate using GTX01077 ALPK1 antibody.

Anti-ALPK1 antibody used in IHC (Paraffin sections) (IHC-P). GTX01077

GTX01077 IHC-P Image

IHC-P analysis of human liver cancer tissue using GTX01077 ALPK1 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed
Dilution : 1:100

REFERENCE

Application Reference

Zhang Y et al. Gut Microbes 2022; 14 (1) : 2038852 Fusobacterium nucleatum promotes colorectal cancer cells adhesion to endothelial cells and facilitates extravasation and metastasis by inducing ALPK1/NF-κB/ICAM1 axis.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399