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ANP antibody [GT1068]

Anti-ANP antibody [GT1068] used in IHC (Paraffin sections) (IHC-P). GTX634219
Anti-ANP antibody [GT1068] used in Western Blot (WB). GTX634219
Anti-ANP antibody [GT1068] used in ELISA (ELISA). GTX634219
Anti-ANP antibody [GT1068] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634219

Cat. No. GTX634219

Host

Mouse

Clonality

Monoclonal

Clone Name

GT1068

Isotype

IgG1

Application

WB, ICC/IF, IHC-P, ELISA, Sandwich ELISA

Reactivity

Human, Mouse
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX634219, Detection: GTX639071

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Predict Reactivity

Rat, Rabbit, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human ANP recombinant protein.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888430

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

natriuretic peptide A , ANF , ANP , ATFB6 , ATRST2 , CDD , CDD-ANF , CDP , PND

Cellular Localization

Secreted

Background

The protein encoded by this gene belongs to the natriuretic peptide family. Natriuretic peptides are implicated in the control of extracellular fluid volume and electrolyte homeostasis. This protein is synthesized as a large precursor (containing a signal peptide), which is processed to release a peptide from the N-terminus with similarity to vasoactive peptide, cardiodilatin, and another peptide from the C-terminus with natriuretic-diuretic activity. Mutations in this gene have been associated with atrial fibrillation familial type 6. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ANP antibody [GT1068] used in IHC (Paraffin sections) (IHC-P). GTX634219

GTX634219 IHC-P Image

ANP antibody [GT1068] detects secreted ANP protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ANP antibody [GT1068] used in Western Blot (WB). GTX634219

GTX634219 WB Image

Mouse tissue extract (50 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with ANP antibody [GT1068] (GTX634219) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ANP antibody [GT1068] used in ELISA (ELISA). GTX634219

GTX634219 ELISA Image

Sandwich ELISA detection of recombinant Human ANP protein, GST tag (GTX109255-pro) using antibodies as below.

Capture: ANP antibody [GT1068] (GTX634219) (5 μg/mL)
Detection: ANP antibody [HL2617] (GTX639071) (1 μg/mL)

Anti-ANP antibody [GT1068] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634219

GTX634219 ICC/IF Image

ANP antibody [GT1068] detects ANP protein at cytoplasm by immunofluorescent analysis.
Sample: SH-SY-5Y cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ANP stained by ANP antibody [GT1068] (GTX634219) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations