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APC antibody [FE9]

Cat. No. GTX16792

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 FE9

Isotype

 IgG1

Application

 WB

Reactivity

 Human, Mouse, Rat
Package
50 μg ($399)
See all APC products

APPLICATION

Application Note

For WB: Use at a concentration of 1 - 1.5μg/ml. Predicted molecular weight: 312 kDa. (SW480 cells, which contain a mutation at codon 1338 of APC and express atruncated APC protein of 147 kDa). Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

312 kDa. ( Note )

Positive Control

HCT116 cells for p300, SW480 cells for truncated APC (p147)

Product Note

Reacts with all full-length and truncated APC proteins

PROPERTIES

Form

Liquid

Buffer

50mM Sodium Phosphate buffer pH7.5, 0.2% Gelatin

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Immunogen used was a synthetic peptide corresponding to the N-terminal 35 amino acid residues of APC.

Purification

Purified by chromatography over Protein G Sepharose followed by FPLC.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

APC regulator of WNT signaling pathway , BTPS2 , DP2 , DP2.5 , DP3 , GS , PPP1R46

Cellular Localization

Cytoplasm, cytoskeleton , Cytoplasm , Cell membrane

Background

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

Database

Research Area

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399