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APEX2 antibody, Internal

Anti-APEX2 antibody, Internal used in Flow cytometry (FACS). GTX80642
Anti-APEX2 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80642
Anti-APEX2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX80642
Anti-APEX2 antibody, Internal used in Western Blot (WB). GTX80642

Cat. No. GTX80642

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
400 μl ($399)
See all APEX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:25
IHC-P 1:10-1:50
FACS 1:10-1:50
Not tested in other applications.

Calculated MW

57 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 143-171 amino acids from the Central region of human APEX2.

Purification

Protein A purified, followed by peptide affinity purification.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 2 , APE2 , APEXL2 , XTH2 , ZGRF2

Cellular Localization

Nucleus,Cytoplasm,Mitochondrion

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes a protein shown to have a weak class II AP endonuclease activity. Most of the encoded protein is located in the nucleus but some is also present in mitochondria. This protein may play an important role in both nuclear and mitochondrial base excision repair. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012]

Database

Research Area

DATA IMAGES

Anti-APEX2 antibody, Internal used in Flow cytometry (FACS). GTX80642

GTX80642 FACS Image

FACS analysis of MCF-7 cells using GTX80642 APEX2 antibody, Internal.
Top histogram : negative control
Bottom histogram : MCF-7 cells

Anti-APEX2 antibody, Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX80642

GTX80642 ICC/IF Image

ICC/IF analysis of U251 cells using GTX80642 APEX2 antibody, Internal.
Green : APEX2
Red : Actin filament
Dilution : 1:25

Anti-APEX2 antibody, Internal used in IHC (Paraffin sections) (IHC-P). GTX80642

GTX80642 IHC-P Image

IHC-P analysis of human lung carcinoma using GTX80642 APEX2 antibody, Internal.

Anti-APEX2 antibody, Internal used in Western Blot (WB). GTX80642

GTX80642 WB Image

WB analysis of MCF-7 whole cell lysate using GTX80642 APEX2 antibody, Internal.
Loading : 20 μg per lane
Dilution : 1:1000

REFERENCE

Application Reference

Md Akram Hossain et al. Front Cell Dev Biol 2021; 9 : 738502 APE2 Is a General Regulator of the ATR-Chk1 DNA Damage Response Pathway to Maintain Genome Integrity in Pancreatic Cancer Cells.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399