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APH1A antibody

Anti-APH1A antibody used in Western Blot (WB). GTX85062

Cat. No. GTX85062

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ELISA

Reactivity

 Human, Mouse
Package
100 μg ($399)
See all APH1A products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5 - 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

RAW 264.7 Cell Lysate(GTX27187)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

APH1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human APH1a.The immunogen is located within amino acids 40 - 90 of APH1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

aph-1 homolog A, gamma-secretase subunit , 6530402N02Rik , APH-1 , APH-1A , CGI-78

Cellular Localization

Endoplasmic reticulum membrane

Background

APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gammasecretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma and epsilonsites and can lead to the accumulation of the Amyloid betapeptide Abetacleavage product that is associated with Alzheimer’s disease. APH1 exists in at least three distinct isoforms with APH1alpha as the principal isoform present in the gammasecretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE.

Database

Research Area

DATA IMAGES

Anti-APH1A antibody used in Western Blot (WB). GTX85062

GTX85062 WB Image

WB analysis of RAW264.7 cell lysate using GTX85062 APH1A antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399