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APS antibody

Anti-APS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82848

Cat. No. GTX82848

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Mouse
Package
100 μg ($399)
See all APS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF 1:250
Not tested in other applications.

Calculated MW

67 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues E(605) A T L G R A R A V E N Q Y S(619) from mouse APS.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SH2B adaptor protein 2 , Aps

Cellular Localization

Cytoplasm,Cell membrane

Background

APS (adapter protein with Pleckstrin homology and Src homology 2 domains) a member of the Lnk family, is an adaptor protein that is involved in B-cell signaling, insulin signaling, cytoskeletal reorganization. It is tyrosine phosphorylated by JAK2, KIT and other kinases during B-cell receptor stimulation of many different cytokines, chemokines and leukokines. APS has been shown to inhibit the JAK-STAT pathway in collaboration with c-Cbl. It is located in the cytoplasm in pre-PDGF (platelet derived growth factor) stimulated cells and localizes to the plasma membrane and peripheral regions upon PDGF stimulation. ? This protein is expressed in B-cells and mast cells when detected in haematopoietic cell lines yet is also expressed in many different tissues including spleen, prostrate, testis, uterus, small intestine and skeletal muscle.

Database

DATA IMAGES

Anti-APS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX82848

GTX82848 ICC/IF Image

ICC/IF analysis of Neuro-2a cells using GTX82848 APS antibody. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Triton X-100 for 10 minute
Dilution : 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399