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ARHGAP25 antibody

Anti-ARHGAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30063
Anti-ARHGAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30063
Anti-ARHGAP25 antibody used in Western Blot (WB). GTX30063
Anti-ARHGAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX30063
Anti-ARHGAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX30063

Cat. No. GTX30063

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399)
See all ARHGAP25 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

73 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-370 of human ARHGAP25 (NP_001159748.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Rho GTPase activating protein 25 , HEL-S-308 , KAIA0053

Background

ARHGAPs, such as ARHGAP25, encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration (Katoh and Katoh, 2004 [PubMed 15254788]).[supplied by OMIM, Mar 2008]

Database

Research Area

DATA IMAGES

Anti-ARHGAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30063

GTX30063 ICC/IF Image

ICC/IF analysis of L929 cells using GTX30063 ARHGAP25 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ARHGAP25 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30063

GTX30063 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX30063 ARHGAP25 antibody.
Blue : DAPI
Dilution : 1:100

Anti-ARHGAP25 antibody used in Western Blot (WB). GTX30063

GTX30063 WB Image

WB analysis of various sample lysates using GTX30063 ARHGAP25 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ARHGAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX30063

GTX30063 IHC-P Image

IHC-P analysis of human gastric tissue using GTX30063 ARHGAP25 antibody.
Dilution : 1:100

Anti-ARHGAP25 antibody used in IHC (Paraffin sections) (IHC-P). GTX30063

GTX30063 IHC-P Image

IHC-P analysis of human kidney tissue using GTX30063 ARHGAP25 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399