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ARID2 antibody

Orthogonal Overexpression
Anti-ARID2 antibody used in Western Blot (WB). GTX129444
Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129444
Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444
Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129444
Anti-ARID2 antibody used in Western Blot (WB). GTX129444

Cat. No. GTX129444

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARID2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

197 kDa. ( Note )

Positive Control

human ARID2-transfected 293T cells , HeLa nuclear , 293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human ARID2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2885998

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

AT-rich interaction domain 2 , BAF200 , CSS6 , p200

Cellular Localization

Nucleus

Background

ARID2 is a subunit of the PBAF chromatin-remodeling complex (see BAF180; MIM 606083), which facilitates ligand-dependent transcriptional activation by nuclear receptors (Yan et al., 2005 [PubMed 15985610]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129444) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129444

GTX129444 ICC/IF Image

ARID2 antibody detects ARID2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ARID2 protein stained by ARID2 antibody (GTX129444) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-ARID2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129444

GTX129444 IHC-P Image

ARID2 antibody detects ARID2 protein on human hepatoma and human normal liver by immunohistochemical analysis.
Sample: (a) Paraffin-embedded human hepatoma (b) Paraffin-embedded human normal liver.
ARID2 antibody (GTX129444) dilution: 1:1000.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

Orthogonal

ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg HeLa whole cell lysate/extract
B. 30 μg HeLa nuclear lysate/extract
5% SDS-PAGE
ARID2 antibody (GTX129444) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

Overexpression

ARID2 antibody detects ARID2 protein by western blot analysis.
A. 30 μg 293T
B. 30 μg of human ARID2-transfected 293T cells
5% SDS-PAGE
ARID2 antibody (GTX129444) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARID2 antibody used in Immunoprecipitation (IP). GTX129444

GTX129444 IP Image

ARID2 antibody immunoprecipitates ARID2 protein in IP experiments.
IP samples: HepG2 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of ARID2 protein by 4 μg ARID2 antibody (GTX129444)
5 % SDS-PAGE
The immunoprecipitated ARID2 protein was detected by ARID2 antibody (GTX129444) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-ARID2 antibody used in Western Blot (WB). GTX129444

GTX129444 WB Image

ARID2 antibody detects ARID2 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ARID2 antibody (GTX129444) diluted by 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169