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ARL1 antibody

Anti-ARL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108258
Anti-ARL1 antibody used in Western Blot (WB). GTX108258
Anti-ARL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108258

Cat. No. GTX108258

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all ARL1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

Positive Control

HUVEC

Predict Reactivity

Mouse, Rat, Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human ARL1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036270

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor like GTPase 1 , ARFL1

Cellular Localization

Golgi apparatus membrane

Background

The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX108258

GTX108258 IHC-P Image

ARL1 antibody detects ARL1 protein at cytoplasm on human liver carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded liver carcinoma.
ARL1 antibody (GTX108258) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARL1 antibody used in Western Blot (WB). GTX108258

GTX108258 WB Image

Sample (30 ug of whole cell lysate)
A: HUVEC
12% SDS PAGE
GTX108258 diluted at 1:1000

Anti-ARL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108258

GTX108258 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using ARL1(GTX108258) antibody at 1:500 dilution.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169