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ARL13B antibody

Orthogonal
Anti-ARL13B antibody used in IHC (Paraffin sections) (IHC-P). GTX122703
Anti-ARL13B antibody used in IHC (Paraffin sections) (IHC-P). GTX122703
Anti-ARL13B antibody used in Western Blot (WB). GTX122703
Anti-ARL13B antibody used in Western Blot (WB). GTX122703
Anti-ARL13B antibody used in Western Blot (WB). GTX122703
Anti-ARL13B antibody used in Western Blot (WB). GTX122703
Anti-ARL13B antibody used in Western Blot (WB). GTX122703
Anti-ARL13B antibody used in Western Blot (WB). GTX122703

Cat. No. GTX122703

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all ARL13B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Positive Control

Mouse liver

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ARL13B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168257

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ADP ribosylation factor like GTPase 13B , ARL2L1 , JBTS8

Background

This gene encodes a member of the ADP-ribosylation factor-like family. The encoded protein is a small GTPase that contains both N-terminal and C-terminal guanine nucleotide-binding motifs. This protein is localized in the cilia and plays a role in cilia formation and in maintenance of cilia. Mutations in this gene are the cause of Joubert syndrome 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ARL13B antibody used in IHC (Paraffin sections) (IHC-P). GTX122703

GTX122703 IHC-P Image

ARL13B antibody detects ARL13B protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ARL13B stained by ARL13B antibody (GTX122703) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ARL13B antibody used in IHC (Paraffin sections) (IHC-P). GTX122703

GTX122703 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ARL13B(GTX122703) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ARL13B antibody used in Western Blot (WB). GTX122703

GTX122703 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ARL13B antibody (GTX122703) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARL13B antibody used in Western Blot (WB). GTX122703

GTX122703 WB Image

Sample (50 μg of whole cell lysate)
A: mouse Liver
10% SDS PAGE
GTX122703 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ARL13B antibody used in Western Blot (WB). GTX122703

GTX122703 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ARL13B antibody (GTX122703) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ARL13B antibody used in Western Blot (WB). GTX122703

GTX122703 WB Image

Orthogonal

The data was published in the journal Theranostics in 2020. PMID: 32042332

Anti-ARL13B antibody used in Western Blot (WB). GTX122703

GTX122703 WB Image

Orthogonal

The data was published in the journal Theranostics in 2020. PMID: 32042332

Anti-ARL13B antibody used in Western Blot (WB). GTX122703

GTX122703 WB Image

Orthogonal

The data was published in the journal Theranostics in 2020. PMID: 32042332

REFERENCE

Application Reference

Li S et al. Theranostics 2020; 10 (4) : 1719-1732 Silica Perturbs Primary Cilia and Causes Myofibroblast Differentiation during Silicosis by Reduction of the KIF3A-Repressor GLI3 Complex.
Application : WB, ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
GTX122703_Proclin.pdf
Package List Price ($)
$ 399
$ 169