APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:5000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
rat kidney , mouse kidney
Predict Reactivity
Bovine, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ARPC2. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949687
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin related protein 2/3 complex subunit 2 , ARC34 , PNAS-139 , PRO2446 , p34-Arc
Cellular Localization
Cytoplasm , cytoskeleton , Cell projection
Background
This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p34 subunit, has yet to be determined. Two alternatively spliced variants have been characterized to date. Additional alternatively spliced variants have been described but their full length nature has not been determined. [provided by RefSeq]
Database
Research Area
DATA IMAGES
|
GTX101984 IHC-P Image
Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ARPC2 antibody (GTX101984) at 1:300 dilution.
|
|
|
GTX101984 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with ARPC2 antibody (GTX101984) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101984 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ARPC2(GTX101984) antibody at 1:200 dilution.
|
|
|
GTX101984 IHC-P Image
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using ARPC2(GTX101984) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
|
|
|
GTX101984 WB Image
ARPC2 antibody detects ARPC2 protein by western blot analysis.
A. 50 μg mouse kidney lysate/extract
10% SDS-PAGE
ARPC2 antibody (GTX101984) dilution: 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101984 WB Image
ARPC2 antibody detects ARPC2 protein by western blot analysis.
A. 50 μg rat kidney lysate/extract
10% SDS-PAGE
ARPC2 antibody (GTX101984) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX101984 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ARPC2 antibody (GTX101984) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
REFERENCE
REVIEW
There are currently no reviews for ARPC2 antibody (GTX101984). Be the first to share your experience with this product.
