APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000 - 1:2000 |
1:100 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein of human ASAH2
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
N-acylsphingosine amidohydrolase 2 , BCDase , HNAC1 , LCDase , N-CDase , NCDase
Cellular Localization
Golgi apparatus membrane,Mitochondrion
Background
Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger (see MIM 603730) (Mitsutake et al., 2001 [PubMed 11328816]).[supplied by OMIM, Mar 2008]
Database
Research Area
DATA IMAGES
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GTX55519 WB Image
WB analysis of various sample lysates using GTX55519 ASAH2 antibody. Dilution : 1:500 Loading : 25μg per lane
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GTX55519 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX55519 ASAH2 antibody.
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GTX55519 IHC-P Image
IHC-P analysis of human stomach tissue using GTX55519 ASAH2 antibody.
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GTX55519 IHC-P Image
IHC-P analysis of rat liver tissue using GTX55519 ASAH2 antibody.
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REFERENCE
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REVIEW
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