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ASIC1 antibody

Anti-ASIC1 antibody used in Western Blot (WB). GTX54804
Anti-ASIC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54804

Cat. No. GTX54804

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-Fr, IP

Reactivity

 Human, Mouse, Rat
Package
50 μl ($639)
See all ASIC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

60 kDa. ( Note )

Product Note

Recognizes isoforms ASIC1α and ASIC1β.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.8 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Rat

Immunogen

Peptide CQKEAKRSSADKGVALSLDD, corresponding to amino acid residues 469-488 (Intracellular, C-terminus) of rat ASIC1 (Accession P55926).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

acid sensing ion channel subunit 1 , Accn2

Cellular Localization

Cell membrane

Background

forms a proton-gated ion channel that may play a role in sensory neuron function and pain perception [RGD, Feb 2006]

Database

Research Area

DATA IMAGES

Anti-ASIC1 antibody used in Western Blot (WB). GTX54804

GTX54804 WB Image

WB analysis of rat brain membrane lysate using GTX54804 ASIC1 antibody preincubated with or without immunogen peptide.
Dilution : 1:200

Anti-ASIC1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX54804

GTX54804 IHC-Fr Image

IHC-Fr analysis of rat brain tissue using GTX54804 ASIC1 antibody.
Panel A : Parvalbumin (PV) positive neurons are shown (green).
Panel B : Neurons with typically enmeshed dendritic trees stain intensely for ASIC1 (red).
Panel C : Double labeling with mouse anti-Parvalbumin (green) reveals strong co-localization of PV with ASIC1.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 639