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ASL antibody

Orthogonal
Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629
Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629
Anti-ASL antibody used in Western Blot (WB). GTX113629
Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629
Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629
Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629
Anti-ASL antibody used in Western Blot (WB). GTX113629

Cat. No. GTX113629

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all ASL products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7

Predict Reactivity

Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.78 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASL. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036281

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

argininosuccinate lyase , ASAL

Background

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629

GTX113629 IHC-P Image

ASL antibody detects ASL protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human hepatocellular carcinoma.
ASL stained by ASL antibody (GTX113629) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629

GTX113629 ICC/IF Image

ASL antibody detects ASL protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASL protein stained by ASL antibody (GTX113629) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-ASL antibody used in Western Blot (WB). GTX113629

GTX113629 WB Image

ASL antibody detects ASL protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
ASL antibody (GTX113629) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629

GTX113629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASL(GTX113629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ASL antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113629

GTX113629 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Argininosuccinate Lyase(GTX113629) antibody at 1:200 dilution.

Anti-ASL antibody used in IHC (Paraffin sections) (IHC-P). GTX113629

GTX113629 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ASL(GTX113629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ASL antibody used in Western Blot (WB). GTX113629

GTX113629 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASL antibody (GTX113629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

Application Reference

Hung YH et al. Oncol Rep 2017; 37 (2) : 969-978 Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells.
Hu L et al. J Biol Chem 2013; 288 (48) : 34599-611 Understanding the role of argininosuccinate lyase transcript variants in the clinical and biochemical variability of the urea cycle disorder argininosuccinic aciduria.
Application : WB
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169