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ATF4 antibody, C-term

Anti-ATF4 antibody, C-term used in Western Blot (WB). GTX89973

Cat. No. GTX89973

Reference 4

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all ATF4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.3-1μg/ml
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89973-PEP).

Product Note

This antibody is expected to recognize both reported isoforms (NP_001666.2; NP_877962.1).

Predict Reactivity

Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence C-EEVRKARGKKRVP, from the C Terminus of the protein sequence according to NP_001666.2; NP_877962.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

RRID

AB_10725702

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

activating transcription factor 4 , CREB-2 , CREB2 , TAXREB67 , TXREB

Cellular Localization

Cytoplasm , Cell membrane , Nucleus

Background

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromosome at q28 in a region containing a large inverted duplication. [provided by RefSeq, Sep 2011]

Database

Research Area

DATA IMAGES

Anti-ATF4 antibody, C-term used in Western Blot (WB). GTX89973

GTX89973 WB Image

WB analysis of K562 lysate using GTX89973 ATF4 antibody, C-term.
Dilution : 0.3μg/ml
Loading : 35μg protein in RIPA buffer

REFERENCE

Application Reference

Xie MX et al. Nat Commun 2021; 12 (1) : 1401 ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking.
Application : WB, IHC-Fr
Reactivity : Mouse
Wu PS et al. PLoS One 2015; 10 (6) : e0130599 Luteolin and Apigenin Attenuate 4-Hydroxy-2-Nonenal-Mediated Cell Death through Modulation of UPR, Nrf2-ARE and MAPK Pathways in PC12 Cells.
Lin MH et al. Toxicology 2014; 315 : 24-37 Lipid peroxidation end product 4-hydroxy-trans-2-nonenal triggers unfolded protein response and heme oxygenase-1 expression in PC12 cells: Roles of ROS and MAPK pathways.
Application : WB
Reactivity : Rat
Weng CY et al. Arch Toxicol 2014; 88 (2) : 213-26 * induces unfolded protein response in vascular endothelial cells.
Application : WB
Reactivity : Mouse
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399