APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat , Raji , PC-12 , Rat-2 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , K562 , THP-1 , HL-60 , HeLa
Predict Reactivity
Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATG3. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2885698
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
autophagy related 3 , APG3 , APG3-LIKE , APG3L , PC3-96
Cellular Localization
Cytoplasm
Background
Autophagy is a process of bulk degradation of cytoplasmic components by the lysosome or vacuole. Human ATG3 displays the same enzymatic characteristics in vitro as yeast Apg3, a protein-conjugating enzyme essential for autophagy (Tanida et al., 2002 [PubMed 11825910]).[supplied by OMIM]
Database
Research Area
DATA IMAGES
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GTX128065 WB Image
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG3 antibody (GTX128065) diluted at 1:1000.
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GTX128065 WB Image
ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:1000
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GTX128065 WB Image
ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg K562 whole cell lysate/extract
B. 30 μg THP-1 whole cell lysate/extract
C. 30 μg HL-60 whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:1000
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GTX128065 WB Image
ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat-2 whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:2000
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GTX128065 WB Image
ATG3 antibody detects ATG3 protein by Western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
B. 30 μg NIH-3T3 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysate/extract
D. 30 μg Raw264.7 whole cell lysate/extract
10 % SDS-PAGE
ATG3 antibody (GTX128065) dilution: 1:1000
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REFERENCE
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REVIEW
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