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ATP citrate lyase antibody [5F8D11]

Anti-ATP citrate lyase antibody [5F8D11] used in Western Blot (WB). GTX60666
Anti-ATP citrate lyase antibody [5F8D11] used in IHC (Paraffin sections) (IHC-P). GTX60666
Anti-ATP citrate lyase antibody [5F8D11] used in IHC (Paraffin sections) (IHC-P). GTX60666
Anti-ATP citrate lyase antibody [5F8D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60666
Anti-ATP citrate lyase antibody [5F8D11] used in ELISA (ELISA). GTX60666
Anti-ATP citrate lyase antibody [5F8D11] used in Flow cytometry (FACS). GTX60666
Anti-ATP citrate lyase antibody [5F8D11] used in Western Blot (WB). GTX60666

Cat. No. GTX60666

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F8D11

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μg ($409)
See all ATP citrate lyase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/50
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

121 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human ATP citrate lyase (AA: 306-502 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP citrate lyase , ACL , ATPCL , CLATP

Cellular Localization

Cytoplasm

Background

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Dec 2014]

Database

Research Area

DATA IMAGES

Anti-ATP citrate lyase antibody [5F8D11] used in Western Blot (WB). GTX60666

GTX60666 WB Image

WB analysis of HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate using GTX60666 ATP citrate lyase antibody [5F8D11].

Anti-ATP citrate lyase antibody [5F8D11] used in IHC (Paraffin sections) (IHC-P). GTX60666

GTX60666 IHC-P Image

IHC-P analysis of esophageal cancer tissue using GTX60666 ATP citrate lyase antibody [5F8D11].

Anti-ATP citrate lyase antibody [5F8D11] used in IHC (Paraffin sections) (IHC-P). GTX60666

GTX60666 IHC-P Image

IHC-P analysis of endometrial cancer tissue using GTX60666 ATP citrate lyase antibody [5F8D11].

Anti-ATP citrate lyase antibody [5F8D11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60666

GTX60666 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60666 ATP citrate lyase antibody [5F8D11].
Green : ATP citrate lyase
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-ATP citrate lyase antibody [5F8D11] used in ELISA (ELISA). GTX60666

GTX60666 ELISA Image

ELISA analysis of antigen using GTX60666 ATP citrate lyase antibody [5F8D11].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-ATP citrate lyase antibody [5F8D11] used in Flow cytometry (FACS). GTX60666

GTX60666 FACS Image

FACS analysis of HeLa cells using GTX60666 ATP citrate lyase antibody [5F8D11].
Green : ATP citrate lyase
Purple : negative control

Anti-ATP citrate lyase antibody [5F8D11] used in Western Blot (WB). GTX60666

GTX60666 WB Image

The data was published in the journal Aging (Albany NY) in 2020.PMID: 32530819

REFERENCE

Application Reference

Wang W et al. Aging (Albany NY) 2020; 12 (12) : 12119-12141 Serum proteomic predicts effectiveness and reveals potential biomarkers for complications in liver transplant patients.
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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409