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ATP5H antibody

Anti-ATP5H antibody used in IHC (Paraffin sections) (IHC-P). GTX32460
Anti-ATP5H antibody used in Western Blot (WB). GTX32460
Anti-ATP5H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32460
Anti-ATP5H antibody used in IHC (Paraffin sections) (IHC-P). GTX32460

Cat. No. GTX32460

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat, Monkey
Package
50 μl ($399)
See all ATP5H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-161 of human ATP5H (NP_006347.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATP synthase peripheral stalk subunit d , APT5H , ATP5H , ATPQ

Cellular Localization

Mitochondrion,Mitochondrion inner membrane

Background

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the d subunit of the Fo complex. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. In addition, three pseudogenes are located on chromosomes 9, 12 and 15. [provided by RefSeq, Jun 2010]

Database

Research Area

DATA IMAGES

Anti-ATP5H antibody used in IHC (Paraffin sections) (IHC-P). GTX32460

GTX32460 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32460 ATP5H antibody.
Dilution : 1:100

Anti-ATP5H antibody used in Western Blot (WB). GTX32460

GTX32460 WB Image

WB analysis of various sample lysates using GTX32460 ATP5H antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-ATP5H antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32460

GTX32460 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX32460 ATP5H antibody.
Blue : DAPI
Dilution : 1:100

Anti-ATP5H antibody used in IHC (Paraffin sections) (IHC-P). GTX32460

GTX32460 IHC-P Image

IHC-P analysis of mouse kidney tissue using GTX32460 ATP5H antibody.
Dilution : 1:100

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399