APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:50-1:200 |
Not tested in other applications.
Calculated MW
Positive Control
HepG2 , 293T
Predict Reactivity
Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.44 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATP6V1A. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2888345
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATPase H+ transporting V1 subunit A , ARCL2D , ATP6A1 , ATP6V1A1 , HO68 , IECEE3 , VA68 , VPP2 , Vma1
Background
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c", and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is one of two V1 domain A subunit isoforms and is found in all tissues. Transcript variants derived from alternative polyadenylation exist. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542](/templates/default/js/lightbox/images/loading.gif "Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542")
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GTX633542 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT3846] (GTX633542) diluted at 1:500.
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GTX633542 FACS Image
ATP6V1A antibody [GT3846] (GTX633542) detects ATP6V1A protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: ATP6V1A antibody [GT3846] (GTX633542) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
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GTX633542 IHC-P Image
ATP6V1A antibody [GT3846] detects ATP6V1A protein at cytoplasm in rat liver by immunohistochemical analysis.
Sample: Paraffin-embedded rat liver.
ATP6V1A antibody [GT3846] (GTX633542) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX633542 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ATP6V1A antibody [GT3846] (GTX633542) diluted at 1:1000.
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GTX633542 IHC-P Image
ATP6V1A antibody [GT3846] detects ATP6V1A protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ATP6V1A antibody [GT3846] (GTX633542) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
There are currently no references for ATP6V1A antibody [GT3846] (GTX633542). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ATP6V1A antibody [GT3846] (GTX633542). Be the first to share your experience with this product.
![Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542](/upload/website/prouct_img/small/GTX633542/GTX633542_42520_20161013_WB_shRNA_watermark_w_23061202_284.webp "Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in Flow cytometry (FACS). GTX633542](/upload/website/prouct_img/small/GTX633542/GTX633542_42520_20181207_FACS_w_23061202_949.webp "Anti-ATP6V1A antibody [GT3846] used in Flow cytometry (FACS). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542](/upload/website/prouct_img/small/GTX633542/GTX633542_42520_20160727_IHC-P_R_w_23061202_381.webp "Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542](/upload/website/prouct_img/small/GTX633542/GTX633542_42520_20160630_WB_w_23061202_881.webp "Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542](/upload/website/prouct_img/small/GTX633542/GTX633542_42520_20160727_IHC-P_M_w_23061202_804.webp "Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542](/upload/website/prouct_img/normal/GTX633542/GTX633542_42520_20161013_WB_shRNA_watermark_w_23061202_284.webp "Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in Flow cytometry (FACS). GTX633542](/upload/website/prouct_img/normal/GTX633542/GTX633542_42520_20181207_FACS_w_23061202_949.webp "Anti-ATP6V1A antibody [GT3846] used in Flow cytometry (FACS). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542](/upload/website/prouct_img/normal/GTX633542/GTX633542_42520_20160727_IHC-P_R_w_23061202_381.webp "Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542](/upload/website/prouct_img/normal/GTX633542/GTX633542_42520_20160630_WB_w_23061202_881.webp "Anti-ATP6V1A antibody [GT3846] used in Western Blot (WB). GTX633542")
![Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542](/upload/website/prouct_img/normal/GTX633542/GTX633542_42520_20160727_IHC-P_M_w_23061202_804.webp "Anti-ATP6V1A antibody [GT3846] used in IHC (Paraffin sections) (IHC-P). GTX633542")