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ATP6V1H antibody [HL1316]

Recombinant antibody Orthogonal
Anti-ATP6V1H antibody [HL1316] used in Western Blot (WB). GTX636734
Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734
Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734
Anti-ATP6V1H antibody [HL1316] used in Western Blot (WB). GTX636734
Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734
Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734

Cat. No. GTX636734

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1316

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Drosophila
Package
100 μl ($399),
25 μl ($169)
See all ATP6V1H products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Predict Reactivity

Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human ATP6V1H. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2910063

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ATPase H+ transporting V1 subunit H , CGI-11 , MSTP042 , NBP1 , SFD , SFDalpha , SFDbeta , VMA13

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular organelles. V-ATPase-dependent organelle acidification is necessary for multiple processes including protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. The encoded protein is the regulatory H subunit of the V1 domain of V-ATPase, which is required for catalysis of ATP but not the assembly of V-ATPase. Decreased expression of this gene may play a role in the development of type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-ATP6V1H antibody [HL1316] used in Western Blot (WB). GTX636734

GTX636734 WB Image

Drosophila tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [HL1316] (GTX636734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734

GTX636734 IHC-P Image

ATP6V1H antibody [HL1316] detects ATP6V1H protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
ATP6V1H stained by ATP6V1H antibody [HL1316] (GTX636734) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734

GTX636734 IHC-P Image

ATP6V1H antibody [HL1316] detects ATP6V1H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse heart.
ATP6V1H stained by ATP6V1H antibody [HL1316] (GTX636734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATP6V1H antibody [HL1316] used in Western Blot (WB). GTX636734

GTX636734 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATP6V1H antibody [HL1316] (GTX636734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734

GTX636734 IHC-P Image

ATP6V1H antibody [HL1316] detects ATP6V1H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ATP6V1H stained by ATP6V1H antibody [HL1316] (GTX636734) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATP6V1H antibody [HL1316] used in IHC (Paraffin sections) (IHC-P). GTX636734

GTX636734 IHC-P Image

ATP6V1H antibody [HL1316] detects ATP6V1H protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse muscle.
ATP6V1H stained by ATP6V1H antibody [HL1316] (GTX636734) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations