APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1,000 |
1:10-1:100 |
1:100 |
Assay dependent |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody has shown cross-reactivity with actin 1, 2, 3, 4, 7, 8, 11 and 12.
PROPERTIES
Form
Liquid
Buffer
Ascites
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Arabidopsis thaliana
Immunogen
Purified Arabidopsis actin protein.
Purification
Unpurified
Conjugation
Unconjugated
RRID
AB_626122
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
actin alpha cardiac muscle 1 , ACTC , ASD5 , CMD1R , CMH11 , LVNC4
Cellular Localization
Cytoplasm, cytoskeleton
Background
Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX80809 ICC/IF Image
ICC/IF analysis of 3T3 cells using GTX80809 Actin antibody [mAbGEa]. Green : Primary antibody Blue : cell nucleus Permeabilization : 0.1% Triton X-100 in TBS for 15 minutes at room temperature Dilution : 1:100 in blocking buffer for at least 1 hour at room temperature
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GTX80809 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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GTX80809 IHC-P Image
IHC-P analysis of human skeletal muscle tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
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GTX80809 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
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GTX80809 IHC-P Image
IHC-P analysis of human colon carcinoma tissue using GTX80809 Actin antibody [mAbGEa]. Left : Primary antibody Right : Negative control without primary antibody Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes Dilution : 1:1000
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GTX80809 ICC/IF Image
ICC/IF analysis of A375 Cells using GTX80809 Actin antibody [mAbGEa]. Cells were probed without (right) or with(left) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : formaldehyde Dilution : 1:20 overnight at 4ºC
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REFERENCE
REVIEW
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